High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation

Keisuke Isobe; Akira Suda; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

doi:10.1364/cleo.2010.cthcc4

High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Department of Electronics and Electrical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore.

Original language	English
Title of host publication	Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference
Subtitle of host publication	2010 Laser Science to Photonic Applications, CLEO/QELS 2010
Publisher	Association for Computing Machinery
ISBN (Print)	9781557528902
DOIs	https://doi.org/10.1364/cleo.2010.cthcc4
Publication status	Published - 2010 Jan 1

Publication series

Name	Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010

ASJC Scopus subject areas

Radiation
Electrical and Electronic Engineering

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10.1364/cleo.2010.cthcc4

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Isobe, K., Suda, A., Hashimoto, H., Kannari, F., Kawano, H., Mizuno, H., Miyawaki, A., & Midorikawa, K. (2010). High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation. In Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010 [5499649] (Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010). Association for Computing Machinery. https://doi.org/10.1364/cleo.2010.cthcc4

High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation. / Isobe, Keisuke; Suda, Akira; Hashimoto, Hiroshi et al.
Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010. Association for Computing Machinery, 2010. 5499649 (Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Isobe, K, Suda, A, Hashimoto, H, Kannari, F, Kawano, H, Mizuno, H, Miyawaki, A & Midorikawa, K 2010, High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation. in Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010., 5499649, Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010, Association for Computing Machinery. https://doi.org/10.1364/cleo.2010.cthcc4

Isobe K, Suda A, Hashimoto H, Kannari F, Kawano H, Mizuno H et al. High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation. In Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010. Association for Computing Machinery. 2010. 5499649. (Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010). doi: 10.1364/cleo.2010.cthcc4

Isobe, Keisuke ; Suda, Akira ; Hashimoto, Hiroshi et al. / High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation. Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010. Association for Computing Machinery, 2010. (Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010).

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AU - Suda, Akira

AU - Hashimoto, Hiroshi

AU - Kannari, Fumihiko

AU - Kawano, Hiroyuki

AU - Mizuno, Hideaki

AU - Miyawaki, Atsushi

AU - Midorikawa, Katsumi

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AB - We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore.

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High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation

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