High-Resolution Plasma Membrane-Selective Imaging by Second Harmonic Generation

Takaha Mizuguchi, Masato Yasui, Mutsuo Nuriya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The plasma membrane is the site of intercellular communication and subsequent intracellular signal transduction. The specific visualization of the plasma membrane in living cells, however, is difficult using fluorescence-based techniques owing to the high background signals from intracellular organelles. In this study, we show that second harmonic generation (SHG)is a high-resolution plasma membrane-selective imaging technique that enables multifaceted investigations of the plasma membrane. In contrast to fluorescence imaging, SHG specifically visualizes the plasma membrane at locations that are not attached to artificial substrates and allows high-resolution imaging because of its subresolution nature. These properties were exploited to measure the distances from the plasma membrane to subcortical actin and tubulin fibers, revealing the precise cytoskeletal organization beneath the plasma membrane. Thus, SHG imaging enables the specific visualization of phenomena at the plasma membrane with unprecedented precision and versatility and should facilitate cell biology research focused on the plasma membrane.

Original languageEnglish
Pages (from-to)359-366
Number of pages8
JournalFood Science and Human Wellness
Volume9
DOIs
Publication statusPublished - 2018 Nov 30

Fingerprint

plasma membrane
Cell Membrane
image analysis
fluorescence
cell communication
Optical Imaging
Tubulin
tubulin
cell biology
Organelles
actin
organelles
Cell Biology
signal transduction
Actins
Signal Transduction
Fluorescence
methodology
Research

Keywords

  • Biological Sciences
  • Biophysics
  • Membrane Architecture

ASJC Scopus subject areas

  • Food Science
  • General

Cite this

High-Resolution Plasma Membrane-Selective Imaging by Second Harmonic Generation. / Mizuguchi, Takaha; Yasui, Masato; Nuriya, Mutsuo.

In: Food Science and Human Wellness, Vol. 9, 30.11.2018, p. 359-366.

Research output: Contribution to journalArticle

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