TY - JOUR
T1 - High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes
AU - Goto-Inoue, Naoko
AU - Hayasaka, Takahiro
AU - Sugiura, Yuki
AU - Taki, Takao
AU - Li, Yu Teh
AU - Matsumoto, Mineo
AU - Setou, Mitsutoshi
N1 - Funding Information:
This work was supported by a Grant-in-Aid under the SENTAN program of the Japan Science and Technology Agency, a Center of Excellence Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology to M.S.; and NIH Grant Number R01NS009626 from the National Institute of Neurological Disorders and Stroke to Y.-T. L.
PY - 2008/7/1
Y1 - 2008/7/1
N2 - Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. Here we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol).
AB - Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. Here we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol).
KW - Glycosphingolipid
KW - Matrix-assisted laser desorption/ionization
KW - Thin-layer chromatography
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U2 - 10.1016/j.jchromb.2008.06.001
DO - 10.1016/j.jchromb.2008.06.001
M3 - Article
C2 - 18571485
AN - SCOPUS:45849095696
SN - 1570-0232
VL - 870
SP - 74
EP - 83
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -