High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes

Naoko Goto-Inoue, Takahiro Hayasaka, Yuki Sugiura, Takao Taki, Yu T. Li, Mineo Matsumoto, Mitsutoshi Setou

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. Here we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol).

Original languageEnglish
Pages (from-to)74-83
Number of pages10
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume870
Issue number1
DOIs
Publication statusPublished - 2008 Jul 1
Externally publishedYes

Fingerprint

Glycosphingolipids
Sensitivity analysis
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Ions
Membranes
Imaging techniques
Sphingosine
Ceramides
Structural analysis
Limit of Detection
Fatty Acids
Staining and Labeling
Technology

Keywords

  • Glycosphingolipid
  • Matrix-assisted laser desorption/ionization
  • Thin-layer chromatography

ASJC Scopus subject areas

  • Biochemistry

Cite this

High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes. / Goto-Inoue, Naoko; Hayasaka, Takahiro; Sugiura, Yuki; Taki, Takao; Li, Yu T.; Matsumoto, Mineo; Setou, Mitsutoshi.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 870, No. 1, 01.07.2008, p. 74-83.

Research output: Contribution to journalArticle

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