High-sensitivity vision restoration via ectopic expression of chimeric rhodopsin in mice

Yusaku Katada, Kazuho Yoshida, Kenta Kobayashi, Hideyuki Okano, Hideki Kandori, Kazuo Tsubota, Toshihide Kurihara

Research output: Contribution to journalArticlepeer-review

Abstract

Photoreception requires amplification by mammalian rhodopsin through G protein activation, which requires a visual cycle. To achieve this in retinal gene therapy, we incorporated human rhodopsin cytoplasmic loops into Gloeobacter rhodopsin, thereby generating Gloeobacter and human chimeric rhodopsin (GHCR). In a murine model of inherited retinal degeneration, we induced retinal GHCR expression by intravitreal injection of a recombinant adeno-associated virus vector. Retinal explant and visual thalamus electrophysiological recordings, behavioral tests, and histological analysis showed that GHCR restored dim-environment vision and prevented the progression of retinal degeneration. Thus, GHCR may be a potent clinical tool for the treatment of retinal disorders.

Original languageEnglish
JournalUnknown Journal
DOIs
Publication statusPublished - 2020 Sep 18

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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