High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates

Kaoru Seiriki, Atsushi Kasai, Takeshi Hashimoto, Wiebke Schulze, Misaki Niu, Shun Yamaguchi, Takanobu Nakazawa, Ken Ichi Inoue, Shiori Uezono, Masahiko Takada, Yuichiro Naka, Hisato Igarashi, Masato Tanuma, James A. Waschek, Yukio Ago, Kenji Tanaka, Atsuko Hayata-Takano, Kazuki Nagayasu, Norihito Shintani, Ryota HashimotoYasuto Kunii, Mizuki Hino, Junya Matsumoto, Hirooki Yabe, Takeharu Nagai, Katsumasa Fujita, Toshio Matsuda, Kazuhiro Takuma, Akemichi Baba, Hitoshi Hashimoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases.

Original languageEnglish
Pages (from-to)1085-1100
Number of pages16
JournalNeuron
Volume94
Issue number6
DOIs
Publication statusPublished - 2017 Jun 21

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Neuroimaging
Primates
Rodentia
Brain
Microscopy
Tomography
Callithrix
Brain Diseases
Fluorescence Microscopy
Animal Models
Light

Keywords

  • cell distribution
  • human post-mortem brain
  • non-human primate brain
  • subcellular resolution
  • whole-brain imaging

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Seiriki, K., Kasai, A., Hashimoto, T., Schulze, W., Niu, M., Yamaguchi, S., ... Hashimoto, H. (2017). High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates. Neuron, 94(6), 1085-1100. https://doi.org/10.1016/j.neuron.2017.05.017

High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates. / Seiriki, Kaoru; Kasai, Atsushi; Hashimoto, Takeshi; Schulze, Wiebke; Niu, Misaki; Yamaguchi, Shun; Nakazawa, Takanobu; Inoue, Ken Ichi; Uezono, Shiori; Takada, Masahiko; Naka, Yuichiro; Igarashi, Hisato; Tanuma, Masato; Waschek, James A.; Ago, Yukio; Tanaka, Kenji; Hayata-Takano, Atsuko; Nagayasu, Kazuki; Shintani, Norihito; Hashimoto, Ryota; Kunii, Yasuto; Hino, Mizuki; Matsumoto, Junya; Yabe, Hirooki; Nagai, Takeharu; Fujita, Katsumasa; Matsuda, Toshio; Takuma, Kazuhiro; Baba, Akemichi; Hashimoto, Hitoshi.

In: Neuron, Vol. 94, No. 6, 21.06.2017, p. 1085-1100.

Research output: Contribution to journalArticle

Seiriki, K, Kasai, A, Hashimoto, T, Schulze, W, Niu, M, Yamaguchi, S, Nakazawa, T, Inoue, KI, Uezono, S, Takada, M, Naka, Y, Igarashi, H, Tanuma, M, Waschek, JA, Ago, Y, Tanaka, K, Hayata-Takano, A, Nagayasu, K, Shintani, N, Hashimoto, R, Kunii, Y, Hino, M, Matsumoto, J, Yabe, H, Nagai, T, Fujita, K, Matsuda, T, Takuma, K, Baba, A & Hashimoto, H 2017, 'High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates', Neuron, vol. 94, no. 6, pp. 1085-1100. https://doi.org/10.1016/j.neuron.2017.05.017
Seiriki K, Kasai A, Hashimoto T, Schulze W, Niu M, Yamaguchi S et al. High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates. Neuron. 2017 Jun 21;94(6):1085-1100. https://doi.org/10.1016/j.neuron.2017.05.017
Seiriki, Kaoru ; Kasai, Atsushi ; Hashimoto, Takeshi ; Schulze, Wiebke ; Niu, Misaki ; Yamaguchi, Shun ; Nakazawa, Takanobu ; Inoue, Ken Ichi ; Uezono, Shiori ; Takada, Masahiko ; Naka, Yuichiro ; Igarashi, Hisato ; Tanuma, Masato ; Waschek, James A. ; Ago, Yukio ; Tanaka, Kenji ; Hayata-Takano, Atsuko ; Nagayasu, Kazuki ; Shintani, Norihito ; Hashimoto, Ryota ; Kunii, Yasuto ; Hino, Mizuki ; Matsumoto, Junya ; Yabe, Hirooki ; Nagai, Takeharu ; Fujita, Katsumasa ; Matsuda, Toshio ; Takuma, Kazuhiro ; Baba, Akemichi ; Hashimoto, Hitoshi. / High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates. In: Neuron. 2017 ; Vol. 94, No. 6. pp. 1085-1100.
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