High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization

Toshiyuki Yoshikawa, Yulan Piao, Jinhui Zhong, Ryo Matoba, Mark G. Carter, Yuxia Wang, Ilya Goldberg, Minoru S.H. Ko

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Mammalian preimplantation embryos provide an excellent opportunity to study temporal and spatial gene expression in whole mount in situ hybridization (WISH). However, large-scale studies are made difficult by the size of the embryos (∼60 μm diameter) and their fragility. We have developed a chamber system that allows parallel processing of embryos without the aid of a microscope. We first selected 91 candidate genes that were transcription factors highly expressed in blastocysts, and more highly expressed in embryonic (ES) than in trophoblast (TS) stem cells. We then used the WISH to identify 48 genes expressed predominantly in the inner cell mass (ICM) and to follow several of these genes in all seven preimplantation stages. The ICM-predominant expressions of these genes suggest their involvement in the pluripotency of embryonic cells. This system provides a useful tool to a systematic genome-scale analysis of preimplantation embryos.

Original languageEnglish
Pages (from-to)213-224
Number of pages12
JournalGene Expression Patterns
Volume6
Issue number2
DOIs
Publication statusPublished - 2006 Jan 1
Externally publishedYes

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Keywords

  • High-throughput screen
  • Hybridization chamber
  • ICM
  • Preimplantation embryo
  • TE
  • Whole mount in situ hybridization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology

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