High-throughput screening of salivary polyamine markers for discrimination of colorectal cancer by multisegment injection capillary electrophoresis tandem mass spectrometry

Kaori Igarashi, Sana Ota, Miku Kaneko, Akiyoshi Hirayama, Masanobu Enomoto, Kenji Katumata, Masahiro Sugimoto, Tomoyoshi Soga

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Polyamine metabolites provide pathophysiological information on disease or therapeutic efficacy, yet rapid screening methods for these biomarkers are lacking. Here, we developed high-throughput polyamine metabolite profiling based on multisegment injection capillary electrophoresis triple quadrupole tandem mass spectrometry (MSI-CE-MS/MS), which allows sequential 40-sample injection followed by electrophoretic separation and specific mass detection. To achieve consecutive analysis of polyamine samples, 1 M formic acid was used as the background electrolyte (BGE). The BGE spacer volume had an apparent effect on peak resolution among samples, and 20 nL was selected as the optimal volume. The use of polyamine isotopomers as the internal standard enabled the correction of matrix effects in MS detection. This method is sensitive, selective and quantitative, and its utility was demonstrated by screening polyamines in 359 salivary samples within 360 min, resulting in discrimination of colorectal cancer patients from noncancer controls.

Original languageEnglish
Article number462355
JournalJournal of Chromatography A
Volume1652
DOIs
Publication statusPublished - 2021 Aug 30

Keywords

  • Biomarker
  • Colorectal cancer
  • Mass spectrometry
  • Multisegment injection
  • Polyamine
  • Saliva
  • capillary electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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