Highly efficient conversion of (±)-mandelic acid to its (R)-(-)-enantiomer by combination of enzyme-mediated oxidation and reduction

Shigeo Tsuchiya; Kenji Miyamoto; Hiromichi Ohta

doi:10.1007/BF01027017

Highly efficient conversion of (±)-mandelic acid to its (R)-(-)-enantiomer by combination of enzyme-mediated oxidation and reduction

Shigeo Tsuchiya, Kenji Miyamoto, Hiromichi Ohta

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

A novel method for total conversion of racemic mandelic acid into its (R)-enantiomer was developed. The method consists of enantioselective oxidation of (S)-(+)-mandelic acid by Alcaligenes bronchisepticus KU 1201 and NADH-dependent asymmetric reduction of resulting benzoylformic acid to (R)-mandelic acid with cell-free extract of Streptococcus faecalis IFO 12964.

Original language	English
Pages (from-to)	1137-1142
Number of pages	6
Journal	Biotechnology Letters
Volume	14
Issue number	12
DOIs	https://doi.org/10.1007/BF01027017
Publication status	Published - 1992 Dec 1

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF01027017

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abstract = "A novel method for total conversion of racemic mandelic acid into its (R)-enantiomer was developed. The method consists of enantioselective oxidation of (S)-(+)-mandelic acid by Alcaligenes bronchisepticus KU 1201 and NADH-dependent asymmetric reduction of resulting benzoylformic acid to (R)-mandelic acid with cell-free extract of Streptococcus faecalis IFO 12964.",

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AU - Tsuchiya, Shigeo

AU - Miyamoto, Kenji

AU - Ohta, Hiromichi

PY - 1992/12/1

Y1 - 1992/12/1

N2 - A novel method for total conversion of racemic mandelic acid into its (R)-enantiomer was developed. The method consists of enantioselective oxidation of (S)-(+)-mandelic acid by Alcaligenes bronchisepticus KU 1201 and NADH-dependent asymmetric reduction of resulting benzoylformic acid to (R)-mandelic acid with cell-free extract of Streptococcus faecalis IFO 12964.

AB - A novel method for total conversion of racemic mandelic acid into its (R)-enantiomer was developed. The method consists of enantioselective oxidation of (S)-(+)-mandelic acid by Alcaligenes bronchisepticus KU 1201 and NADH-dependent asymmetric reduction of resulting benzoylformic acid to (R)-mandelic acid with cell-free extract of Streptococcus faecalis IFO 12964.

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ER -

Highly efficient conversion of (±)-mandelic acid to its (R)-(-)-enantiomer by combination of enzyme-mediated oxidation and reduction

Abstract

ASJC Scopus subject areas

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