Highly efficient conversion of (±)-mandelic acid to its (R)-(-)-enantiomer by combination of enzyme-mediated oxidation and reduction

Shigeo Tsuchiya; Kenji Miyamoto; Hiromichi Ohta

doi:10.1007/BF01027017

Highly efficient conversion of (±)-mandelic acid to its (R)-(-)-enantiomer by combination of enzyme-mediated oxidation and reduction

Shigeo Tsuchiya, Kenji Miyamoto, Hiromichi Ohta

Department of Biosciences and Informatics

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44 Citations (Scopus)

Abstract

A novel method for total conversion of racemic mandelic acid into its (R)-enantiomer was developed. The method consists of enantioselective oxidation of (S)-(+)-mandelic acid by Alcaligenes bronchisepticus KU 1201 and NADH-dependent asymmetric reduction of resulting benzoylformic acid to (R)-mandelic acid with cell-free extract of Streptococcus faecalis IFO 12964.

Original language	English
Pages (from-to)	1137-1142
Number of pages	6
Journal	Biotechnology Letters
Volume	14
Issue number	12
DOIs	https://doi.org/10.1007/BF01027017
Publication status	Published - 1992 Dec 1

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ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Cite this

Tsuchiya, S., Miyamoto, K., & Ohta, H. (1992). Highly efficient conversion of (±)-mandelic acid to its (R)-(-)-enantiomer by combination of enzyme-mediated oxidation and reduction. Biotechnology Letters, 14(12), 1137-1142. https://doi.org/10.1007/BF01027017

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10.1007/BF01027017