Highly efficient conversion of (±)-mandelic acid to its (R)-(-)-enantiomer by combination of enzyme-mediated oxidation and reduction

Shigeo Tsuchiya, Kenji Miyamoto, Hiromichi Ohta

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A novel method for total conversion of racemic mandelic acid into its (R)-enantiomer was developed. The method consists of enantioselective oxidation of (S)-(+)-mandelic acid by Alcaligenes bronchisepticus KU 1201 and NADH-dependent asymmetric reduction of resulting benzoylformic acid to (R)-mandelic acid with cell-free extract of Streptococcus faecalis IFO 12964.

Original languageEnglish
Pages (from-to)1137-1142
Number of pages6
JournalBiotechnology Letters
Issue number12
Publication statusPublished - 1992 Dec 1


ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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