Highly selective hydroxylation of benzene to phenol by wild-type cytochrome P450BM3 assisted by decoy molecules

Osami Shoji; Tatsuya Kunimatsu; Norifumi Kawakami; Yoshihito Watanabe

doi:10.1002/anie.201300282

Highly selective hydroxylation of benzene to phenol by wild-type cytochrome P450BM3 assisted by decoy molecules

Osami Shoji, Tatsuya Kunimatsu, Norifumi Kawakami, Yoshihito Watanabe

Research output: Contribution to journal › Article › peer-review

98 Citations (Scopus)

Abstract

Playing tricks on enzymes: Direct hydroxylation of benzene to phenol was catalyzed by wild-type P450BM3 in the presence of perfluorinated carboxylic acids as decoy molecules. The catalytic turnover rate reached 120 min ^-1 per P450. The selectivity towards phenol production was very high and no overoxidation products were detected.

Original language	English
Pages (from-to)	6606-6610
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	26
DOIs	https://doi.org/10.1002/anie.201300282
Publication status	Published - 2013 Jun 24
Externally published	Yes

Keywords

carboxylic acid
cytochromes
decoy molecule
hydroxylation

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201300282

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abstract = "Playing tricks on enzymes: Direct hydroxylation of benzene to phenol was catalyzed by wild-type P450BM3 in the presence of perfluorinated carboxylic acids as decoy molecules. The catalytic turnover rate reached 120 min -1 per P450. The selectivity towards phenol production was very high and no overoxidation products were detected.",

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AU - Kawakami, Norifumi

AU - Watanabe, Yoshihito

PY - 2013/6/24

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AB - Playing tricks on enzymes: Direct hydroxylation of benzene to phenol was catalyzed by wild-type P450BM3 in the presence of perfluorinated carboxylic acids as decoy molecules. The catalytic turnover rate reached 120 min -1 per P450. The selectivity towards phenol production was very high and no overoxidation products were detected.

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KW - cytochromes

KW - decoy molecule

KW - hydroxylation

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ER -

Highly selective hydroxylation of benzene to phenol by wild-type cytochrome P450BM3 assisted by decoy molecules

Abstract

Keywords

ASJC Scopus subject areas

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