Highly selective hydroxylation of benzene to phenol by wild-type cytochrome P450BM3 assisted by decoy molecules

Osami Shoji; Tatsuya Kunimatsu; Norifumi Kawakami; Yoshihito Watanabe

doi:10.1002/anie.201300282

Highly selective hydroxylation of benzene to phenol by wild-type cytochrome P450BM3 assisted by decoy molecules

Osami Shoji, Tatsuya Kunimatsu, Norifumi Kawakami, Yoshihito Watanabe

Research output: Contribution to journal › Article

71 Citations (Scopus)

Abstract

Playing tricks on enzymes: Direct hydroxylation of benzene to phenol was catalyzed by wild-type P450BM3 in the presence of perfluorinated carboxylic acids as decoy molecules. The catalytic turnover rate reached 120 min ^-1 per P450. The selectivity towards phenol production was very high and no overoxidation products were detected.

Original language	English
Pages (from-to)	6606-6610
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	26
DOIs	https://doi.org/10.1002/anie.201300282
Publication status	Published - 2013 Jun 24
Externally published	Yes

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Keywords

carboxylic acid
cytochromes
decoy molecule
hydroxylation

ASJC Scopus subject areas

Catalysis
Chemistry(all)

Cite this

Shoji, O., Kunimatsu, T., Kawakami, N., & Watanabe, Y. (2013). Highly selective hydroxylation of benzene to phenol by wild-type cytochrome P450BM3 assisted by decoy molecules. Angewandte Chemie - International Edition, 52(26), 6606-6610. https://doi.org/10.1002/anie.201300282

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Access to Document

10.1002/anie.201300282