Highly sensitive and rapid sequential cortisol detection using twin sensor QCM

Takeshi Ito, Nobuyoshi Aoki, Satoru Kaneko, Koji Suzuki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We present a rapid cortisol detecting system with the integration of flow injection analysis in order to adapt sequential analysis. The detecting system consists of a twin sensor chip on quartz crystal micro balance (QCM) method. One sensor monitored antigen-antibody interaction, together with the other sensor as a reference to remove environmental influences. A competitive assay was performed on monitoring the antigen-antibody interaction using cortisol coupled with bovine serum albumin (Crt-BSA) as a tracer. The binding between antigen and antibody could be uncoupled with a glycine-NaOH solution to adapt sequential analysis. One detection cycle, including regeneration step was performed within 10 minutes at a flow rate of 6 μL min-1. The sensor system could quantitatively detect cortisol levels ranging from 5 pg mL-1 to 100 pg mL-1. These results indicated the sensor system has the potential to be used for active stress-monitoring and in the diagnosis of disease.

Original languageEnglish
Pages (from-to)7469-7474
Number of pages6
JournalAnalytical Methods
Volume6
Issue number18
DOIs
Publication statusPublished - 2014 Sep 21

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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