Highly sensitive fluorescence detection of avidin/streptavidin with an optical interference mirror slide

Mitsuru Yasuda, Takuo Akimoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This paper presents highly sensitive fluorescence detections of avidin and streptavidin using an optical interference mirror (OIM) slide consisting of a plane mirror covered with an optical interference layer. Compared with a common glass slide, the OIM slide can enhance the fluorescence from a dye by more than 100-fold. We fabricated an OIM slide by depositing an optical interference layer of Al 2 O 3 on an Ag mirror. To enhance the fluorescence maximally, the optimal thickness of the Al 2 O 3 layer was estimated from optical interference theory. For detections of protein, avidin/streptavidin labeled with fluorescein, Cy3, and Cy5 were detected with biotin immobilized on an OIM slide with the optimal Al 2 O 3 thickness. We achieved a sensitivity improvement of more than 50-fold, comparing with a glass slide. Such a high degree of improvement would be a significant contribution to further progress in biomedical research and medical diagnostics. 2012

Original languageEnglish
Pages (from-to)947-952
Number of pages6
Journalanalytical sciences
Volume28
Issue number10
DOIs
Publication statusPublished - 2012 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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