TY - JOUR
T1 - Highly sensitive fluorescence detection of avidin/streptavidin with an optical interference mirror slide
AU - Yasuda, Mitsuru
AU - Akimoto, Takuo
N1 - Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - This paper presents highly sensitive fluorescence detections of avidin and streptavidin using an optical interference mirror (OIM) slide consisting of a plane mirror covered with an optical interference layer. Compared with a common glass slide, the OIM slide can enhance the fluorescence from a dye by more than 100-fold. We fabricated an OIM slide by depositing an optical interference layer of Al 2 O 3 on an Ag mirror. To enhance the fluorescence maximally, the optimal thickness of the Al 2 O 3 layer was estimated from optical interference theory. For detections of protein, avidin/streptavidin labeled with fluorescein, Cy3, and Cy5 were detected with biotin immobilized on an OIM slide with the optimal Al 2 O 3 thickness. We achieved a sensitivity improvement of more than 50-fold, comparing with a glass slide. Such a high degree of improvement would be a significant contribution to further progress in biomedical research and medical diagnostics. 2012
AB - This paper presents highly sensitive fluorescence detections of avidin and streptavidin using an optical interference mirror (OIM) slide consisting of a plane mirror covered with an optical interference layer. Compared with a common glass slide, the OIM slide can enhance the fluorescence from a dye by more than 100-fold. We fabricated an OIM slide by depositing an optical interference layer of Al 2 O 3 on an Ag mirror. To enhance the fluorescence maximally, the optimal thickness of the Al 2 O 3 layer was estimated from optical interference theory. For detections of protein, avidin/streptavidin labeled with fluorescein, Cy3, and Cy5 were detected with biotin immobilized on an OIM slide with the optimal Al 2 O 3 thickness. We achieved a sensitivity improvement of more than 50-fold, comparing with a glass slide. Such a high degree of improvement would be a significant contribution to further progress in biomedical research and medical diagnostics. 2012
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U2 - 10.2116/analsci.28.947
DO - 10.2116/analsci.28.947
M3 - Article
C2 - 23059989
AN - SCOPUS:84871178923
VL - 28
SP - 947
EP - 952
JO - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 10
ER -