Abstract
This paper presents highly sensitive fluorescence detections of avidin and streptavidin using an optical interference mirror (OIM) slide consisting of a plane mirror covered with an optical interference layer. Compared with a common glass slide, the OIM slide can enhance the fluorescence from a dye by more than 100-fold. We fabricated an OIM slide by depositing an optical interference layer of Al 2 O 3 on an Ag mirror. To enhance the fluorescence maximally, the optimal thickness of the Al 2 O 3 layer was estimated from optical interference theory. For detections of protein, avidin/streptavidin labeled with fluorescein, Cy3, and Cy5 were detected with biotin immobilized on an OIM slide with the optimal Al 2 O 3 thickness. We achieved a sensitivity improvement of more than 50-fold, comparing with a glass slide. Such a high degree of improvement would be a significant contribution to further progress in biomedical research and medical diagnostics. 2012
| Original language | English |
|---|---|
| Pages (from-to) | 947-952 |
| Number of pages | 6 |
| Journal | analytical sciences |
| Volume | 28 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2012 Jan 1 |
| Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry