Hindgut Bacterial Flora Analysis in Oriental Honey Buzzard (Pernis ptilorhynchus)

Kazuya Nagai, Ken Ichi Tokita, Hirotake Ono, Kiyoshi Uchida, Fumio Sakamoto, Hiroyoshi Higuchi

Research output: Contribution to journalArticle

Abstract

The intestinal microbiome is known to affect host health through various effects on nutrition and immunity. The oriental honey buzzard (OHB) is a raptor that feeds on bees and wasps. Due to its restricted diet, we reasoned that the OHB may have a unique microbiome. The aim of this study was to characterize the structure of the intestinal flora of oriental honey buzzards and to investigate the difference of intestinal bacterial flora between individuals in the wild and those reared in captivity. We investigated the intestinal microbiome of seven wild buzzards (Wild), one zoo-reared (Zoo), and one individual reared in captivity for one month (Rearing). Average operational taxonomic units in Wild and Rearing were 69.4 and 113, respectively. Diversity indices such as ACE, Chao 1, Shannon, and Alpha were significantly lower in the Wild than in the Rearing samples. These results suggest that the variety of Wild microbiome is remarkably low. At the phylum level, the composition of the microbiome was similar in all three groups, with firmicutes and bacteroidetes predominating. The third most abundant bacterium in Wild was Proteobacteria, whereas it was Actinobacteria in Rearing and unclassified bacteria in Zoo. Thus, microbiome composition is affected even with just one month of human rearing.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalZoological Science
Volume36
Issue number1
DOIs
Publication statusPublished - 2019 Feb 1

Fingerprint

Pernis
Pernis apivorus
hindgut
flora
rearing
zoos
Firmicutes
Actinobacteria
Proteobacteria
bacteria
birds of prey
intestinal microorganisms
microbiome
Apoidea
immunity
nutrition

Keywords

  • 16S rRNA
  • microbiome
  • Oriental honey buzzard
  • pyrosequencing
  • rearing artifact

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Hindgut Bacterial Flora Analysis in Oriental Honey Buzzard (Pernis ptilorhynchus). / Nagai, Kazuya; Tokita, Ken Ichi; Ono, Hirotake; Uchida, Kiyoshi; Sakamoto, Fumio; Higuchi, Hiroyoshi.

In: Zoological Science, Vol. 36, No. 1, 01.02.2019, p. 77-81.

Research output: Contribution to journalArticle

Nagai, K, Tokita, KI, Ono, H, Uchida, K, Sakamoto, F & Higuchi, H 2019, 'Hindgut Bacterial Flora Analysis in Oriental Honey Buzzard (Pernis ptilorhynchus)', Zoological Science, vol. 36, no. 1, pp. 77-81. https://doi.org/10.2108/zs180121
Nagai, Kazuya ; Tokita, Ken Ichi ; Ono, Hirotake ; Uchida, Kiyoshi ; Sakamoto, Fumio ; Higuchi, Hiroyoshi. / Hindgut Bacterial Flora Analysis in Oriental Honey Buzzard (Pernis ptilorhynchus). In: Zoological Science. 2019 ; Vol. 36, No. 1. pp. 77-81.
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