Hippocampal activity during serotonergic neuronal activity manipulation using optogenetics

D. Kondo, Yasue Mitsukura, K. Yoshida, I. Tsutsui-Kimura, N. Takata, Kenji Tanaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In order to improve brain dysfunction such as mental disease, it is necessary to clarify the mechanism of activity in the brain. To clarify the relationship between the brain region and the neurotransmitter is important for elucidating the brain. Especially investigating the relationship between serotonin and hippocampus leads to elucidate of the mechanism of depression and sleep. The technique called optogenetics enabled only specific cells to be manipulated by light stimulation in recent years. This technique is a useful technique for clarifying the function of the brain. Because instead of stimulating multiple cells such as electrical stimulation, optogenetics can stimulate only specific cells, so that its function can be clarified. This study focused on neurotransmitter serotonin and the brain region called the hippocampus. For these purpose we can clarify the relationship between serotonin and hippocampus by manipulating serotonin neural activity using optogenetics while invasively measuring the ventral hippocampus of the mouse. In addition, identification of pyramidal cell layer is necessary to clarify hippocampal activity. Currently, visual estimation is performed based on the histology that dissected the brain, it still has a problem. Now this technique can't estimate ambiguous of the brain part and measure in an online manner. For this problem, we focus on the clarity of the brain part estimation when we used optogenetics. Therefore, we could estimate the pyramidal cell layer of the hippocampus by analyzing nerve activity and thought that the effect of serotonin activation or suppression on the hippocampus could be clarified. As a result, we showed that pyramidal cell layer can be identified by using principal component analysis, and could clarify the variation of nerve activity in the pyramidal cell layer of hippocampus during serotonin operation.

Original languageEnglish
Title of host publication2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-6
Number of pages6
Volume2018-January
ISBN (Electronic)9781538648735
DOIs
Publication statusPublished - 2018 Jan 12
Event2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Philadelphia, United States
Duration: 2017 Dec 2 → …

Other

Other2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017
CountryUnited States
CityPhiladelphia
Period17/12/2 → …

Fingerprint

Optogenetics
Brain
Hippocampus
Serotonin
Pyramidal Cells
Cells
Neurotransmitter Agents
Histology
Principal Component Analysis
Principal component analysis
Electric Stimulation
Sleep
Chemical activation
Depression

ASJC Scopus subject areas

  • Health Informatics
  • Clinical Neurology
  • Signal Processing
  • Cardiology and Cardiovascular Medicine

Cite this

Kondo, D., Mitsukura, Y., Yoshida, K., Tsutsui-Kimura, I., Takata, N., & Tanaka, K. (2018). Hippocampal activity during serotonergic neuronal activity manipulation using optogenetics. In 2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Proceedings (Vol. 2018-January, pp. 1-6). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SPMB.2017.8257057

Hippocampal activity during serotonergic neuronal activity manipulation using optogenetics. / Kondo, D.; Mitsukura, Yasue; Yoshida, K.; Tsutsui-Kimura, I.; Takata, N.; Tanaka, Kenji.

2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Proceedings. Vol. 2018-January Institute of Electrical and Electronics Engineers Inc., 2018. p. 1-6.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kondo, D, Mitsukura, Y, Yoshida, K, Tsutsui-Kimura, I, Takata, N & Tanaka, K 2018, Hippocampal activity during serotonergic neuronal activity manipulation using optogenetics. in 2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Proceedings. vol. 2018-January, Institute of Electrical and Electronics Engineers Inc., pp. 1-6, 2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017, Philadelphia, United States, 17/12/2. https://doi.org/10.1109/SPMB.2017.8257057
Kondo D, Mitsukura Y, Yoshida K, Tsutsui-Kimura I, Takata N, Tanaka K. Hippocampal activity during serotonergic neuronal activity manipulation using optogenetics. In 2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Proceedings. Vol. 2018-January. Institute of Electrical and Electronics Engineers Inc. 2018. p. 1-6 https://doi.org/10.1109/SPMB.2017.8257057
Kondo, D. ; Mitsukura, Yasue ; Yoshida, K. ; Tsutsui-Kimura, I. ; Takata, N. ; Tanaka, Kenji. / Hippocampal activity during serotonergic neuronal activity manipulation using optogenetics. 2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Proceedings. Vol. 2018-January Institute of Electrical and Electronics Engineers Inc., 2018. pp. 1-6
@inproceedings{ed968a98e6064dc0986ab9f822a842a3,
title = "Hippocampal activity during serotonergic neuronal activity manipulation using optogenetics",
abstract = "In order to improve brain dysfunction such as mental disease, it is necessary to clarify the mechanism of activity in the brain. To clarify the relationship between the brain region and the neurotransmitter is important for elucidating the brain. Especially investigating the relationship between serotonin and hippocampus leads to elucidate of the mechanism of depression and sleep. The technique called optogenetics enabled only specific cells to be manipulated by light stimulation in recent years. This technique is a useful technique for clarifying the function of the brain. Because instead of stimulating multiple cells such as electrical stimulation, optogenetics can stimulate only specific cells, so that its function can be clarified. This study focused on neurotransmitter serotonin and the brain region called the hippocampus. For these purpose we can clarify the relationship between serotonin and hippocampus by manipulating serotonin neural activity using optogenetics while invasively measuring the ventral hippocampus of the mouse. In addition, identification of pyramidal cell layer is necessary to clarify hippocampal activity. Currently, visual estimation is performed based on the histology that dissected the brain, it still has a problem. Now this technique can't estimate ambiguous of the brain part and measure in an online manner. For this problem, we focus on the clarity of the brain part estimation when we used optogenetics. Therefore, we could estimate the pyramidal cell layer of the hippocampus by analyzing nerve activity and thought that the effect of serotonin activation or suppression on the hippocampus could be clarified. As a result, we showed that pyramidal cell layer can be identified by using principal component analysis, and could clarify the variation of nerve activity in the pyramidal cell layer of hippocampus during serotonin operation.",
author = "D. Kondo and Yasue Mitsukura and K. Yoshida and I. Tsutsui-Kimura and N. Takata and Kenji Tanaka",
year = "2018",
month = "1",
day = "12",
doi = "10.1109/SPMB.2017.8257057",
language = "English",
volume = "2018-January",
pages = "1--6",
booktitle = "2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Proceedings",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

TY - GEN

T1 - Hippocampal activity during serotonergic neuronal activity manipulation using optogenetics

AU - Kondo, D.

AU - Mitsukura, Yasue

AU - Yoshida, K.

AU - Tsutsui-Kimura, I.

AU - Takata, N.

AU - Tanaka, Kenji

PY - 2018/1/12

Y1 - 2018/1/12

N2 - In order to improve brain dysfunction such as mental disease, it is necessary to clarify the mechanism of activity in the brain. To clarify the relationship between the brain region and the neurotransmitter is important for elucidating the brain. Especially investigating the relationship between serotonin and hippocampus leads to elucidate of the mechanism of depression and sleep. The technique called optogenetics enabled only specific cells to be manipulated by light stimulation in recent years. This technique is a useful technique for clarifying the function of the brain. Because instead of stimulating multiple cells such as electrical stimulation, optogenetics can stimulate only specific cells, so that its function can be clarified. This study focused on neurotransmitter serotonin and the brain region called the hippocampus. For these purpose we can clarify the relationship between serotonin and hippocampus by manipulating serotonin neural activity using optogenetics while invasively measuring the ventral hippocampus of the mouse. In addition, identification of pyramidal cell layer is necessary to clarify hippocampal activity. Currently, visual estimation is performed based on the histology that dissected the brain, it still has a problem. Now this technique can't estimate ambiguous of the brain part and measure in an online manner. For this problem, we focus on the clarity of the brain part estimation when we used optogenetics. Therefore, we could estimate the pyramidal cell layer of the hippocampus by analyzing nerve activity and thought that the effect of serotonin activation or suppression on the hippocampus could be clarified. As a result, we showed that pyramidal cell layer can be identified by using principal component analysis, and could clarify the variation of nerve activity in the pyramidal cell layer of hippocampus during serotonin operation.

AB - In order to improve brain dysfunction such as mental disease, it is necessary to clarify the mechanism of activity in the brain. To clarify the relationship between the brain region and the neurotransmitter is important for elucidating the brain. Especially investigating the relationship between serotonin and hippocampus leads to elucidate of the mechanism of depression and sleep. The technique called optogenetics enabled only specific cells to be manipulated by light stimulation in recent years. This technique is a useful technique for clarifying the function of the brain. Because instead of stimulating multiple cells such as electrical stimulation, optogenetics can stimulate only specific cells, so that its function can be clarified. This study focused on neurotransmitter serotonin and the brain region called the hippocampus. For these purpose we can clarify the relationship between serotonin and hippocampus by manipulating serotonin neural activity using optogenetics while invasively measuring the ventral hippocampus of the mouse. In addition, identification of pyramidal cell layer is necessary to clarify hippocampal activity. Currently, visual estimation is performed based on the histology that dissected the brain, it still has a problem. Now this technique can't estimate ambiguous of the brain part and measure in an online manner. For this problem, we focus on the clarity of the brain part estimation when we used optogenetics. Therefore, we could estimate the pyramidal cell layer of the hippocampus by analyzing nerve activity and thought that the effect of serotonin activation or suppression on the hippocampus could be clarified. As a result, we showed that pyramidal cell layer can be identified by using principal component analysis, and could clarify the variation of nerve activity in the pyramidal cell layer of hippocampus during serotonin operation.

UR - http://www.scopus.com/inward/record.url?scp=85050521742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050521742&partnerID=8YFLogxK

U2 - 10.1109/SPMB.2017.8257057

DO - 10.1109/SPMB.2017.8257057

M3 - Conference contribution

AN - SCOPUS:85050521742

VL - 2018-January

SP - 1

EP - 6

BT - 2017 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2017 - Proceedings

PB - Institute of Electrical and Electronics Engineers Inc.

ER -