Histamine H1-receptors on astrocytes in primary cultures: A possible target for histaminergic neurones

H. Fukui, N. Inagaki, S. Ito, Akiharu Kubo, H. Kondoh, A. Yamatodani, H. Wada

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The characteristics of histamine H1-receptors expressed on astrocytes from the cerebral cortex of new born rats were analysed by the [3H]-mepyramine binding assay. The appartent dissociatiokn constant (Kd) was 10.4 nM and the binding capacity (Bmax) was 262 fmol/mg proteinl. H1-antagonists inhibited the [3H]mepyramine bindings and the isomers of chlorpheniramine showed a stereoselectivity for the inhibition of the bindings. Two distinct populations of cultured astrocytes, type-1 and type-2 astrocytes, were enriched and histamine-induced accumulations of inositol phosphates (IP) and cyclic AMP and histamine-evoked Ca++ signals were examined. Histamine stimulated the accumulation of IP in type-2 astrocytes, but not in type-1 astrocytes. The accumulation of cyclic AMP induced by histamine was observed in type-1 astrocytes, although not in type-2 astrocytes. Histamine-induced Ca++ signals were observed in 17.2% of type-1 astrocytes and in 72.9% of type-2 astrocytes. Histamine-induced Ca++ signals in type-2 astrocytes were antagonized by H1-antagonists, but not by H2- antagonists. Histamine-induced Ca++ signals were classified into 4 patterns, ie. transient, oscillatory, sustained and biphasic. When extracellular Ca++ was omitted or La+++ was added to the extracellular medium, sustained phase of Ca++ signal disappeared and transient and oscillatory patterns were only observed. Phorbol ester inhibited histamineinduced Ca++ signals but pertussis toxin (IAP) and orgnanic voltage dependent CA++ channel blockers had no effect. Histamine-induced Ca++ elevation appeared initially in processes and then Ca++ wave propagated to the cell soma. Ca++ elevation was observed only in the processes in some cells.

Original languageEnglish
Pages (from-to)161-180
Number of pages20
JournalAgents and Actions
Volume33
Issue numberSUPPL.
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Histamine H1 Receptors
Astrocytes
Neurons
Histamine
Pyrilamine
Cyclic AMP
Chlorpheniramine
Stereoselectivity
Inositol Phosphates
Pertussis Toxin
Carisoprodol
Phorbol Esters
Isomers
Cerebral Cortex
Rats
Assays
Phosphates

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

Cite this

Fukui, H., Inagaki, N., Ito, S., Kubo, A., Kondoh, H., Yamatodani, A., & Wada, H. (1991). Histamine H1-receptors on astrocytes in primary cultures: A possible target for histaminergic neurones. Agents and Actions, 33(SUPPL.), 161-180.

Histamine H1-receptors on astrocytes in primary cultures : A possible target for histaminergic neurones. / Fukui, H.; Inagaki, N.; Ito, S.; Kubo, Akiharu; Kondoh, H.; Yamatodani, A.; Wada, H.

In: Agents and Actions, Vol. 33, No. SUPPL., 1991, p. 161-180.

Research output: Contribution to journalArticle

Fukui, H, Inagaki, N, Ito, S, Kubo, A, Kondoh, H, Yamatodani, A & Wada, H 1991, 'Histamine H1-receptors on astrocytes in primary cultures: A possible target for histaminergic neurones', Agents and Actions, vol. 33, no. SUPPL., pp. 161-180.
Fukui, H. ; Inagaki, N. ; Ito, S. ; Kubo, Akiharu ; Kondoh, H. ; Yamatodani, A. ; Wada, H. / Histamine H1-receptors on astrocytes in primary cultures : A possible target for histaminergic neurones. In: Agents and Actions. 1991 ; Vol. 33, No. SUPPL. pp. 161-180.
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