Histamine-induced cyclic AMP accumulation in type-1 and type-2 astrocytes in primary culture

Akiharu Kubo, Hiroyuki Fukui, Naoyuki Inagaki, Akira Kanamura, Hiroshi Wada

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Histamine-induced cyclic AMP (cAMP) accumulation was studied in purified primary cultures of type-1 and type-2 astrocytes from neonatal rat brain. Histamine induced remarkable cAMP accumulation in type-1 astrocytes in a dose-dependent manner (EC50 = 1.2 × 10-5 M, Emax = 1100% of control). In contrast, histamine had no significant effect on cAMP accumulation in type-2 astrocytes. Famotidine, an H2-antagonist, dose-dependently inhibited histamine-induced cAMP accumulation in type-1 astrocytes (Ki = 3 × 10-8 M), but mepyramine (10-6 M), an H1-antagonist, had no effect. Dimaprit and impromidine, H2-agonists, stimulated cAMP accumulation, but 2-pyridylethylamine, an H1-agonist, did not stimulate it nor augment the H2-agonist-induced cAMP accumulation. These results indicate that (1) histamine induces cAMP accumulation in type-1 astrocytes but not in type-2 astrocytes, and that (2) histamine-induced cAMP accumulation in type-1 astrocytes is mediated by H2-receptors without significant augmentation via H1-receptors.

Original languageEnglish
Pages (from-to)249-253
Number of pages5
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume208
Issue number3
DOIs
Publication statusPublished - 1991 Nov 13
Externally publishedYes

Keywords

  • (Rat)
  • Astrocytes (type 1)
  • Histamine
  • Histamine H receptor
  • cAMP

ASJC Scopus subject areas

  • Pharmacology

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