Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats

Shinzo Kato, Hiromasa Ishii, Sadakazu Aiso, Shuji Yamashita, Daisuke Ito, Masaharu Tsuchiya

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Abstract

The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti-alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol.

Original languageEnglish
Pages (from-to)66-69
Number of pages4
JournalHepatology
Volume12
Issue number1
Publication statusPublished - 1990 Jul

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Alcohol Dehydrogenase
Liver
Methylphenazonium Methosulfate
Glutaral
Formaldehyde
Alcohols
Staining and Labeling
Antibodies
Enzymes

ASJC Scopus subject areas

  • Hepatology

Cite this

Kato, S., Ishii, H., Aiso, S., Yamashita, S., Ito, D., & Tsuchiya, M. (1990). Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats. Hepatology, 12(1), 66-69.

Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats. / Kato, Shinzo; Ishii, Hiromasa; Aiso, Sadakazu; Yamashita, Shuji; Ito, Daisuke; Tsuchiya, Masaharu.

In: Hepatology, Vol. 12, No. 1, 07.1990, p. 66-69.

Research output: Contribution to journalArticle

Kato, S, Ishii, H, Aiso, S, Yamashita, S, Ito, D & Tsuchiya, M 1990, 'Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats', Hepatology, vol. 12, no. 1, pp. 66-69.
Kato, Shinzo ; Ishii, Hiromasa ; Aiso, Sadakazu ; Yamashita, Shuji ; Ito, Daisuke ; Tsuchiya, Masaharu. / Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats. In: Hepatology. 1990 ; Vol. 12, No. 1. pp. 66-69.
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