Histoculture drug response assay to monitor chemoresponse.

Shinji Ohie; Yasuhiro Udagawa; Daisuke Aoki; Shiro Nozawa

Histoculture drug response assay to monitor chemoresponse.

Shinji Ohie, Yasuhiro Udagawa, Daisuke Aoki, Shiro Nozawa

Research output: Contribution to journal › Article › peer-review

Abstract

We provide a detailed explanation of the procedure of the histoculture drug response assay (HDRA) with 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) end point among several modified HDRA procedures. Fresh surgical specimens are cut into approx 1- to 2-mm3 pieces and put on a gelatin sponge infiltrated with culture medium containing a test drug. After incubation for 7 d, cell viability is assessed by the MTT assay. HDRA uses cancer tissue fragments with cells growing in three dimensions, with maintenance of intercellular contact and interactions with stromal cells. Therefore, it seems that HDRA can assess the sensitivity of tumor cells to anticancer drugs in conditions similar to those in vivo and, consequently, shows high prediction rate.

Original language	English
Pages (from-to)	79-86
Number of pages	8
Journal	Methods in molecular medicine
Volume	110
Publication status	Published - 2005
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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AU - Ohie, Shinji

AU - Udagawa, Yasuhiro

AU - Aoki, Daisuke

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AB - We provide a detailed explanation of the procedure of the histoculture drug response assay (HDRA) with 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) end point among several modified HDRA procedures. Fresh surgical specimens are cut into approx 1- to 2-mm3 pieces and put on a gelatin sponge infiltrated with culture medium containing a test drug. After incubation for 7 d, cell viability is assessed by the MTT assay. HDRA uses cancer tissue fragments with cells growing in three dimensions, with maintenance of intercellular contact and interactions with stromal cells. Therefore, it seems that HDRA can assess the sensitivity of tumor cells to anticancer drugs in conditions similar to those in vivo and, consequently, shows high prediction rate.

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AN - SCOPUS:33644634559

SN - 1543-1894

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JO - Methods in molecular medicine

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ER -

Histoculture drug response assay to monitor chemoresponse.

Abstract

ASJC Scopus subject areas

UN SDGs

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