Histological and electrophysiological analysis of the corticospinal pathway to forelimb motoneurons in common marmosets

Takahiro Kondo, Yamato Yoshihara, Kimika Yoshino-Saito, Tomofumi Sekiguchi, Akito Kosugi, Yuta Miyazaki, Yukio Nishimura, Hirotaka J. Okano, Masaya Nakamura, Hideyuki Okano, Tadashi Isa, Junichi Ushiba

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Using histological and electrophysiological methods, we identified the neuroanatomical properties of the common marmoset corticospinal tract (CST), which underlies hand/arm motor control. Biotinylated dextran amine (BDA) was injected into the primary motor cortex to anterogradely label CST axons in the cervical segments, revealing that most CST axons descend in the contralateral dorsolateral funiculus (DLF; 85.0%), and some in the ipsilateral DLF (10.7%). Terminal buttons were mainly found in the contralateral lamina VII of the gray matter, but projection to lamina IX, where forelimb motoneurons are located, was rare. Bilateral projections were more abundant than found in the rat CST, resembling the CST organization of other primates. Intracellular recordings were made from 57 forelimb motoneurons on the contralateral side to stimulation, which revealed no monosynaptic excitatory postsynaptic potentials (EPSPs), but di- or polysynaptic EPSPs and inhibitory synaptic potentials were commonly found. Local field potentials showed monosynaptic excitation mainly in laminae VII, where abundant BDA-labeled CST terminals were observed. These results suggest that direct corticomotoneuronal projection is absent in common marmosets but di- or oligosynaptic effects would be mediated by spinal interneurons.

Original languageEnglish
Pages (from-to)35-44
Number of pages10
JournalNeuroscience Research
Volume98
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Callithrix
Pyramidal Tracts
Forelimb
Motor Neurons
Excitatory Postsynaptic Potentials
Axons
Synaptic Potentials
Motor Cortex
Interneurons
Primates
Arm
Hand

Keywords

  • Common marmoset
  • Corticospinal tract
  • Manual dexterity
  • Motoneuron
  • Non-human primate
  • Segmental interneuron

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Histological and electrophysiological analysis of the corticospinal pathway to forelimb motoneurons in common marmosets. / Kondo, Takahiro; Yoshihara, Yamato; Yoshino-Saito, Kimika; Sekiguchi, Tomofumi; Kosugi, Akito; Miyazaki, Yuta; Nishimura, Yukio; Okano, Hirotaka J.; Nakamura, Masaya; Okano, Hideyuki; Isa, Tadashi; Ushiba, Junichi.

In: Neuroscience Research, Vol. 98, 01.09.2015, p. 35-44.

Research output: Contribution to journalArticle

Kondo, Takahiro ; Yoshihara, Yamato ; Yoshino-Saito, Kimika ; Sekiguchi, Tomofumi ; Kosugi, Akito ; Miyazaki, Yuta ; Nishimura, Yukio ; Okano, Hirotaka J. ; Nakamura, Masaya ; Okano, Hideyuki ; Isa, Tadashi ; Ushiba, Junichi. / Histological and electrophysiological analysis of the corticospinal pathway to forelimb motoneurons in common marmosets. In: Neuroscience Research. 2015 ; Vol. 98. pp. 35-44.
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