Histological responses of cutaneous vascular lesions following photodynamic therapy with Talaporfin sodium: A chicken comb model

Takafumi Ohshiro, Tatsuo Nakajima, Hisao Ogata, Kazuo Kishi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Mono-L-aspartyl chlorin e6 (Talaporfin sodium) is a novel photosensitizer, and is currently being used in photodynamic therapy for various malignant tumors in combination with irradiation with a 664 nm laser. An interesting characteristic of Talaporfin sodium is that the skin photosensitivity after injection of this agent disappears faster than any other existing photosensitizers. This study examined the vascular events that occurred postirradiation in the chicken comb as a capillary malformation model after photosensitization with Talaporfin sodium. Materials and Methods: A single intravenous bolus injections of Talaporfin sodium was administered to the chickens, and a 1 cm diameter area of the comb of each animal was irradiated with a 664 nm visible red laser. The gross changes in the chicken combs were recorded for 7-14 days after photodynamic therapy. For the histological examination, HE, PTAH and Azan stained sections were analyzed. Results: All treated chicken combs had blanched after photodynamic therapy. Microscopy demonstrated an absence of erythrocytes and the vessel lumina were obliterated, leaving the normal overlying epidermis completely intact. Concomitantly with selective destruction of the capillaries in the target area, moderate invasion of inflammatory cells and a slight increase in the stroma were observed. Conclusions: In the chicken comb model, photodynamic therapy with Talaporfin sodium effectively achieved selective destruction of the microvasculature while leaving the epidermis intact. Our results strongly suggest that photodynamic therapy with Talaporfin sodium could be a feasible method to treat dermal hypervascular lesions.

Original languageEnglish
Pages (from-to)176-184
Number of pages9
JournalKeio Journal of Medicine
Volume58
Issue number3
DOIs
Publication statusPublished - 2009 Sep

Fingerprint

Comb and Wattles
Photochemotherapy
Blood Vessels
Chickens
Skin
Photosensitizing Agents
Epidermis
Lasers
Photosensitivity Disorders
Microvessels
Talaporfin
Intravenous Injections
Microscopy
Erythrocytes
Injections

Keywords

  • Mono-L-aspartyl chlorin e6
  • Photodynamic therapy
  • Port-wine stains
  • Talaporfin sodium
  • Vascular occlusion

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Histological responses of cutaneous vascular lesions following photodynamic therapy with Talaporfin sodium : A chicken comb model. / Ohshiro, Takafumi; Nakajima, Tatsuo; Ogata, Hisao; Kishi, Kazuo.

In: Keio Journal of Medicine, Vol. 58, No. 3, 09.2009, p. 176-184.

Research output: Contribution to journalArticle

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