Histopathological characteristics of hypervascular cholangiocellular carcinoma as an early stage of cholangiocellular carcinoma

Yuya Sato, Hidenori Ojima, Hiroaki Onaya, Taisuke Mori, Nobuyoshi Hiraoka, Yoji Kishi, Satoshi Nara, Minoru Esaki, Kazuaki Shimada, Tomoo Kosuge, Kenich Sugihara, Yae Kanai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aim: Prognosis of hypervascular cholangiocellular carcinoma (h-CCC) is reportedly better than that of ordinary hypovascular CCC (o-CCC). The aim of this study is to clarify the histopathological characteristics of h-CCC. Methods: On the basis of the findings in the arterial phase of contrast-enhanced computed tomography, 16 cases of mass-forming-type CCC were divided into two groups (h-CCC, n=8; o-CCC, n=8). Areas of high (Area H-a) and low (Area H-b) attenuation in h-CCC cases and areas of low attenuation in o-CCC cases (Area O) were delineated. These areas were then evaluated histopathologically to determine the proportion of tumor cells, fibrous stroma, arterial vessel density, and immunohistochemical expression of Vascular endothelial growth factor angiopoietin-2; cytokeratin 7, CK19, SOX9 and SOX17 genes; epithelial cell adhesion molecule and the Bmi-1, Ki-67, epithelial membrane antigen and polyclonal carcinoembryonic antigen. Results: The areal ratio of tumor cells decreased and that of fibrous stroma increased in the following order: Area H-a, Area H-b and Area O. Values for AVD and neural cell adhesion molecule positivity rate were significantly higher in Area H-a than in Areas H-b or O. Expressions of vascular endothelial growth factor and angiopoietin-2 were significantly higher in Areas H-a and H-b than in Area O. The Ki-67 labeling index increased in the following order: Area H-a, Area H-b and Area O. Conclusion: A high areal ratio of tumor cells and AVD as well as a high expression of stem cells and angiogenic markers were observed in cases of h-CCC, whereas the areal ratio of fibrous stroma and malignant potential were low. These results suggest that h-CCC may represent the early stage of CCC.

Original languageEnglish
Pages (from-to)1119-1129
Number of pages11
JournalHepatology Research
Volume44
Issue number11
DOIs
Publication statusPublished - 2014 Oct 1
Externally publishedYes

Fingerprint

Cholangiocarcinoma
Angiopoietin-2
Vascular Endothelial Growth Factor A
Keratin-7
Neural Cell Adhesion Molecules
Mucin-1
Neoplasms
Carcinoembryonic Antigen
Stem Cells
Tomography
Genes

Keywords

  • Angiogenesis
  • Arterial vessel density
  • Cholangiocellular carcinoma
  • Dynamic computed tomography
  • Immunohistochemistry
  • Stem cell marker

ASJC Scopus subject areas

  • Hepatology
  • Infectious Diseases

Cite this

Histopathological characteristics of hypervascular cholangiocellular carcinoma as an early stage of cholangiocellular carcinoma. / Sato, Yuya; Ojima, Hidenori; Onaya, Hiroaki; Mori, Taisuke; Hiraoka, Nobuyoshi; Kishi, Yoji; Nara, Satoshi; Esaki, Minoru; Shimada, Kazuaki; Kosuge, Tomoo; Sugihara, Kenich; Kanai, Yae.

In: Hepatology Research, Vol. 44, No. 11, 01.10.2014, p. 1119-1129.

Research output: Contribution to journalArticle

Sato, Y, Ojima, H, Onaya, H, Mori, T, Hiraoka, N, Kishi, Y, Nara, S, Esaki, M, Shimada, K, Kosuge, T, Sugihara, K & Kanai, Y 2014, 'Histopathological characteristics of hypervascular cholangiocellular carcinoma as an early stage of cholangiocellular carcinoma', Hepatology Research, vol. 44, no. 11, pp. 1119-1129. https://doi.org/10.1111/hepr.12236
Sato, Yuya ; Ojima, Hidenori ; Onaya, Hiroaki ; Mori, Taisuke ; Hiraoka, Nobuyoshi ; Kishi, Yoji ; Nara, Satoshi ; Esaki, Minoru ; Shimada, Kazuaki ; Kosuge, Tomoo ; Sugihara, Kenich ; Kanai, Yae. / Histopathological characteristics of hypervascular cholangiocellular carcinoma as an early stage of cholangiocellular carcinoma. In: Hepatology Research. 2014 ; Vol. 44, No. 11. pp. 1119-1129.
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abstract = "Aim: Prognosis of hypervascular cholangiocellular carcinoma (h-CCC) is reportedly better than that of ordinary hypovascular CCC (o-CCC). The aim of this study is to clarify the histopathological characteristics of h-CCC. Methods: On the basis of the findings in the arterial phase of contrast-enhanced computed tomography, 16 cases of mass-forming-type CCC were divided into two groups (h-CCC, n=8; o-CCC, n=8). Areas of high (Area H-a) and low (Area H-b) attenuation in h-CCC cases and areas of low attenuation in o-CCC cases (Area O) were delineated. These areas were then evaluated histopathologically to determine the proportion of tumor cells, fibrous stroma, arterial vessel density, and immunohistochemical expression of Vascular endothelial growth factor angiopoietin-2; cytokeratin 7, CK19, SOX9 and SOX17 genes; epithelial cell adhesion molecule and the Bmi-1, Ki-67, epithelial membrane antigen and polyclonal carcinoembryonic antigen. Results: The areal ratio of tumor cells decreased and that of fibrous stroma increased in the following order: Area H-a, Area H-b and Area O. Values for AVD and neural cell adhesion molecule positivity rate were significantly higher in Area H-a than in Areas H-b or O. Expressions of vascular endothelial growth factor and angiopoietin-2 were significantly higher in Areas H-a and H-b than in Area O. The Ki-67 labeling index increased in the following order: Area H-a, Area H-b and Area O. Conclusion: A high areal ratio of tumor cells and AVD as well as a high expression of stem cells and angiogenic markers were observed in cases of h-CCC, whereas the areal ratio of fibrous stroma and malignant potential were low. These results suggest that h-CCC may represent the early stage of CCC.",
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AU - Ojima, Hidenori

AU - Onaya, Hiroaki

AU - Mori, Taisuke

AU - Hiraoka, Nobuyoshi

AU - Kishi, Yoji

AU - Nara, Satoshi

AU - Esaki, Minoru

AU - Shimada, Kazuaki

AU - Kosuge, Tomoo

AU - Sugihara, Kenich

AU - Kanai, Yae

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N2 - Aim: Prognosis of hypervascular cholangiocellular carcinoma (h-CCC) is reportedly better than that of ordinary hypovascular CCC (o-CCC). The aim of this study is to clarify the histopathological characteristics of h-CCC. Methods: On the basis of the findings in the arterial phase of contrast-enhanced computed tomography, 16 cases of mass-forming-type CCC were divided into two groups (h-CCC, n=8; o-CCC, n=8). Areas of high (Area H-a) and low (Area H-b) attenuation in h-CCC cases and areas of low attenuation in o-CCC cases (Area O) were delineated. These areas were then evaluated histopathologically to determine the proportion of tumor cells, fibrous stroma, arterial vessel density, and immunohistochemical expression of Vascular endothelial growth factor angiopoietin-2; cytokeratin 7, CK19, SOX9 and SOX17 genes; epithelial cell adhesion molecule and the Bmi-1, Ki-67, epithelial membrane antigen and polyclonal carcinoembryonic antigen. Results: The areal ratio of tumor cells decreased and that of fibrous stroma increased in the following order: Area H-a, Area H-b and Area O. Values for AVD and neural cell adhesion molecule positivity rate were significantly higher in Area H-a than in Areas H-b or O. Expressions of vascular endothelial growth factor and angiopoietin-2 were significantly higher in Areas H-a and H-b than in Area O. The Ki-67 labeling index increased in the following order: Area H-a, Area H-b and Area O. Conclusion: A high areal ratio of tumor cells and AVD as well as a high expression of stem cells and angiogenic markers were observed in cases of h-CCC, whereas the areal ratio of fibrous stroma and malignant potential were low. These results suggest that h-CCC may represent the early stage of CCC.

AB - Aim: Prognosis of hypervascular cholangiocellular carcinoma (h-CCC) is reportedly better than that of ordinary hypovascular CCC (o-CCC). The aim of this study is to clarify the histopathological characteristics of h-CCC. Methods: On the basis of the findings in the arterial phase of contrast-enhanced computed tomography, 16 cases of mass-forming-type CCC were divided into two groups (h-CCC, n=8; o-CCC, n=8). Areas of high (Area H-a) and low (Area H-b) attenuation in h-CCC cases and areas of low attenuation in o-CCC cases (Area O) were delineated. These areas were then evaluated histopathologically to determine the proportion of tumor cells, fibrous stroma, arterial vessel density, and immunohistochemical expression of Vascular endothelial growth factor angiopoietin-2; cytokeratin 7, CK19, SOX9 and SOX17 genes; epithelial cell adhesion molecule and the Bmi-1, Ki-67, epithelial membrane antigen and polyclonal carcinoembryonic antigen. Results: The areal ratio of tumor cells decreased and that of fibrous stroma increased in the following order: Area H-a, Area H-b and Area O. Values for AVD and neural cell adhesion molecule positivity rate were significantly higher in Area H-a than in Areas H-b or O. Expressions of vascular endothelial growth factor and angiopoietin-2 were significantly higher in Areas H-a and H-b than in Area O. The Ki-67 labeling index increased in the following order: Area H-a, Area H-b and Area O. Conclusion: A high areal ratio of tumor cells and AVD as well as a high expression of stem cells and angiogenic markers were observed in cases of h-CCC, whereas the areal ratio of fibrous stroma and malignant potential were low. These results suggest that h-CCC may represent the early stage of CCC.

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KW - Dynamic computed tomography

KW - Immunohistochemistry

KW - Stem cell marker

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