Histopathological features and prognostic significance of the micropapillary pattern in lung adenocarcinoma

Kazunori Kamiya, Yuichiro Hayashi, Junya Douguchi, Akinori Hashiguchi, Taketo Yamada, Yotaro Izumi, Masazumi Watanabe, Masafumi Kawamura, Hirohisa Horinouchi, Naoki Shimada, Koichi Kobayashi, Michiie Sakamoto

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Abstract

The micropapillary pattern is characterized by small papillary tufts with no fibrovascular core lying in spaces and has been reported as an aggressive variant of carcinoma in several organs. We investigated the histopathobiological properties of the micropapillary pattern with immunohistochemistry, serial sections, and electron microscopy in lung adenocarcinoma. We further analyzed its clinicopathological character and prognosis. The subjects included 383 adenocarcinoma cases, of which 184 (48%) were micropapillary pattern-positive and 199 (52%) were micropapillary pattern-negative. On histology, micropapillary tufts seemed to float in the alveolar space or spaces encased by connective tissues, whereas serial sections revealed that most tufts had continuity with other tufts and even with the main tumor. Positive staining for the adhesion molecules E-cadherin and β-catenin suggested the preservation of tight adhesion, and electron microscopy showed the existence of intercellular junctions. Negative staining for laminin and loss of basement membrane as determined by electron microscopy suggest a loss of cell-matrix contact. Positive staining for Ki-67 indicates that cells constituting micropapillary tufts retained their proliferation potency. There were no CD34-positive cells in micropapillary tufts, and the loss of the vascular core was confirmed. In micropapillary pattern-positive cases, lymphatic invasion was identified significantly more frequently than in micropapillary pattern-negative cases (P<0.001), even at stageIA (without lymph node metastasis, N=197) (P<0.001). The 5-year and 10-year overall survival rates of the micropapillary pattern-positive stageIA group were 77.6 and 67.6%, respectively, which were significantly less than those of the micropapillary pattern-negative stageIA group (98.1 and 98.1%) (P<0.001). In conclusion, cells constituting the micropapillary pattern are likely to have acquired anchorage-independent growth and a potential for high malignancy.

Original languageEnglish
Pages (from-to)992-1001
Number of pages10
JournalModern Pathology
Volume21
Issue number8
DOIs
Publication statusPublished - 2008 Aug

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Electron Microscopy
Staining and Labeling
Catenins
Negative Staining
Intercellular Junctions
Laminin
Cadherins
Basement Membrane
Connective Tissue
Blood Vessels
Neoplasms
Histology
Adenocarcinoma
Lymph Nodes
Immunohistochemistry
Neoplasm Metastasis
Carcinoma
Adenocarcinoma of lung
Growth

Keywords

  • Electron microscopy
  • Histopathology
  • Immunohistochemistry
  • Lung adenocarcinoma
  • Micropapillary pattern
  • Prognosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Histopathological features and prognostic significance of the micropapillary pattern in lung adenocarcinoma. / Kamiya, Kazunori; Hayashi, Yuichiro; Douguchi, Junya; Hashiguchi, Akinori; Yamada, Taketo; Izumi, Yotaro; Watanabe, Masazumi; Kawamura, Masafumi; Horinouchi, Hirohisa; Shimada, Naoki; Kobayashi, Koichi; Sakamoto, Michiie.

In: Modern Pathology, Vol. 21, No. 8, 08.2008, p. 992-1001.

Research output: Contribution to journalArticle

Kamiya, K, Hayashi, Y, Douguchi, J, Hashiguchi, A, Yamada, T, Izumi, Y, Watanabe, M, Kawamura, M, Horinouchi, H, Shimada, N, Kobayashi, K & Sakamoto, M 2008, 'Histopathological features and prognostic significance of the micropapillary pattern in lung adenocarcinoma', Modern Pathology, vol. 21, no. 8, pp. 992-1001. https://doi.org/10.1038/modpathol.2008.79
Kamiya, Kazunori ; Hayashi, Yuichiro ; Douguchi, Junya ; Hashiguchi, Akinori ; Yamada, Taketo ; Izumi, Yotaro ; Watanabe, Masazumi ; Kawamura, Masafumi ; Horinouchi, Hirohisa ; Shimada, Naoki ; Kobayashi, Koichi ; Sakamoto, Michiie. / Histopathological features and prognostic significance of the micropapillary pattern in lung adenocarcinoma. In: Modern Pathology. 2008 ; Vol. 21, No. 8. pp. 992-1001.
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