TY - JOUR
T1 - HLA class I and class II expression of pulmonary adenocarcinoma cells and the influence of interferon gamma
AU - Yano, Tokujiro
AU - Fukuyama, Yasuro
AU - Yokoyama, Hideki
AU - Kuninaka, Shinji
AU - Asoh, Hiroshi
AU - Katsuda, Yasaburo
AU - Ichinose, Yukito
PY - 1998/6/1
Y1 - 1998/6/1
N2 - Background: The clinico-biological significance of HLA (both class I antigen and class II one) expressed on tumor cells still remains controversial. Methods: Tumor cells were freshly separated from 33 surgical specimens of pulmonary adenocarcinoma. The tumor cells were incubated for 24 h in the presence or absence of IFN-γ (130 International Units/ml). After incubation, the cells were cytocentrifuged onto glass slides and immunostained with either an anti-HLA class I (A, B, C) monoclonal antibody or anti-HLA class II (DR) one. Results: In 22 of 33 cases (66.7%), the HLA class I were individually expressed by more than 60% of tumor cells while so were the HLA class II in 15 (45.4%). No significant correlation was observed between the HLA class I expression and the HLA class II one. The proportion of HLA class I-positive tumor cells correlated with neither the grade of histological differentiation nor the stage of disease. In contrast, the proportion of HLA class II-positive tumor cells correlated with both the grade of histological differentiation and the stage. In most cases, IFN-γ was found to increase the proportion of class II-positive tumor cells as well as that of class I-positive cells. Conclusions: The above findings thus suggested that the HLA class II expression might therefore represent a manifestation of cellular differentiation and that IFN-γ may, as a result, have the potential to differentiate cancer cells. Copyright (C) 1998 Elsevier Science Ireland Ltd.
AB - Background: The clinico-biological significance of HLA (both class I antigen and class II one) expressed on tumor cells still remains controversial. Methods: Tumor cells were freshly separated from 33 surgical specimens of pulmonary adenocarcinoma. The tumor cells were incubated for 24 h in the presence or absence of IFN-γ (130 International Units/ml). After incubation, the cells were cytocentrifuged onto glass slides and immunostained with either an anti-HLA class I (A, B, C) monoclonal antibody or anti-HLA class II (DR) one. Results: In 22 of 33 cases (66.7%), the HLA class I were individually expressed by more than 60% of tumor cells while so were the HLA class II in 15 (45.4%). No significant correlation was observed between the HLA class I expression and the HLA class II one. The proportion of HLA class I-positive tumor cells correlated with neither the grade of histological differentiation nor the stage of disease. In contrast, the proportion of HLA class II-positive tumor cells correlated with both the grade of histological differentiation and the stage. In most cases, IFN-γ was found to increase the proportion of class II-positive tumor cells as well as that of class I-positive cells. Conclusions: The above findings thus suggested that the HLA class II expression might therefore represent a manifestation of cellular differentiation and that IFN-γ may, as a result, have the potential to differentiate cancer cells. Copyright (C) 1998 Elsevier Science Ireland Ltd.
KW - Differentiation
KW - HLA class I
KW - HLA class II
KW - IFN-γ
KW - Immunocytochemistry
KW - Pulmonary adenocarcinoma
KW - Stage of the disease
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U2 - 10.1016/S0169-5002(98)00010-5
DO - 10.1016/S0169-5002(98)00010-5
M3 - Article
C2 - 9733053
AN - SCOPUS:0031818878
VL - 20
SP - 185
EP - 190
JO - Lung Cancer
JF - Lung Cancer
SN - 0169-5002
IS - 3
ER -