HMGB proteins function as universal sentinels for nucleic-acid-mediated innate immune responses

Hideyuki Yanai, Tatsuma Ban, Zhichao Wang, Myoung Kwon Choi, Takeshi Kawamura, Hideo Negishi, Makoto Nakasato, Yan Lu, Sho Hangai, Ryuji Koshiba, David Savitsky, Lorenza Ronfani, Shizuo Akira, Marco E. Bianchi, Kenya Honda, Tomohiko Tamura, Tatsuhiko Kodama, Tadatsugu Taniguchi

Research output: Contribution to journalArticle

417 Citations (Scopus)

Abstract

The activation of innate immune responses by nucleic acids is crucial to protective and pathological immunities and is mediated by the transmembrane Toll-like receptors (TLRs) and cytosolic receptors. However, it remains unknown whether a mechanism exists that integrates these nucleic-acid-sensing systems. Here we show that high-mobility group box (HMGB) proteins 1, 2 and 3 function as universal sentinels for nucleic acids. HMGBs bind to all immunogenic nucleic acids examined with a correlation between affinity and immunogenic potential. Hmgb1-/-and Hmgb2-/-mouse cells are defective in type-I interferon and inflammatory cytokine induction by DNA or RNA targeted to activate the cytosolic nucleic-acid-sensing receptors; cells in which the expression of all three HMGBs is suppressed show a more profound defect, accompanied by impaired activation of the transcription factors interferon regulatory factor 3 (IRF3) and nuclear factor (NF)-B. The absence of HMGBs also severely impairs the activation of TLR3, TLR7 and TLR9 by their cognate nucleic acids. Our results therefore indicate a hierarchy in the nucleic-acid-mediated activation of immune responses, wherein the selective activation of nucleic-acid-sensing receptors is contingent on the more promiscuous sensing of nucleic acids by HMGBs. These findings may have implications for understanding the evolution of the innate immune system and for the treatment of immunological disorders.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalNature
Volume462
Issue number7269
DOIs
Publication statusPublished - 2009 Nov 5
Externally publishedYes

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HMGB Proteins
Innate Immunity
Nucleic Acids
HMGB3 Protein
HMGB2 Protein
Interferon Regulatory Factor-3
HMGB1 Protein
cyhalothrin
Interferon Type I
Toll-Like Receptors
Immune System
Immunity
Transcription Factors

ASJC Scopus subject areas

  • General

Cite this

Yanai, H., Ban, T., Wang, Z., Choi, M. K., Kawamura, T., Negishi, H., ... Taniguchi, T. (2009). HMGB proteins function as universal sentinels for nucleic-acid-mediated innate immune responses. Nature, 462(7269), 99-103. https://doi.org/10.1038/nature08512

HMGB proteins function as universal sentinels for nucleic-acid-mediated innate immune responses. / Yanai, Hideyuki; Ban, Tatsuma; Wang, Zhichao; Choi, Myoung Kwon; Kawamura, Takeshi; Negishi, Hideo; Nakasato, Makoto; Lu, Yan; Hangai, Sho; Koshiba, Ryuji; Savitsky, David; Ronfani, Lorenza; Akira, Shizuo; Bianchi, Marco E.; Honda, Kenya; Tamura, Tomohiko; Kodama, Tatsuhiko; Taniguchi, Tadatsugu.

In: Nature, Vol. 462, No. 7269, 05.11.2009, p. 99-103.

Research output: Contribution to journalArticle

Yanai, H, Ban, T, Wang, Z, Choi, MK, Kawamura, T, Negishi, H, Nakasato, M, Lu, Y, Hangai, S, Koshiba, R, Savitsky, D, Ronfani, L, Akira, S, Bianchi, ME, Honda, K, Tamura, T, Kodama, T & Taniguchi, T 2009, 'HMGB proteins function as universal sentinels for nucleic-acid-mediated innate immune responses', Nature, vol. 462, no. 7269, pp. 99-103. https://doi.org/10.1038/nature08512
Yanai H, Ban T, Wang Z, Choi MK, Kawamura T, Negishi H et al. HMGB proteins function as universal sentinels for nucleic-acid-mediated innate immune responses. Nature. 2009 Nov 5;462(7269):99-103. https://doi.org/10.1038/nature08512
Yanai, Hideyuki ; Ban, Tatsuma ; Wang, Zhichao ; Choi, Myoung Kwon ; Kawamura, Takeshi ; Negishi, Hideo ; Nakasato, Makoto ; Lu, Yan ; Hangai, Sho ; Koshiba, Ryuji ; Savitsky, David ; Ronfani, Lorenza ; Akira, Shizuo ; Bianchi, Marco E. ; Honda, Kenya ; Tamura, Tomohiko ; Kodama, Tatsuhiko ; Taniguchi, Tadatsugu. / HMGB proteins function as universal sentinels for nucleic-acid-mediated innate immune responses. In: Nature. 2009 ; Vol. 462, No. 7269. pp. 99-103.
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