Hochuekkito, a Japanese Herbal Medicine, Restores Metabolic Homeostasis between Mitochondrial and Glycolytic Pathways Impaired by Influenza A Virus Infection

Keita Takanashi, Katsuaki Dan, Sho Kanzaki, Hideki Hasegawa, Kenji Watanabe, Kaoru Ogawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Hochuekkito (HKT), a traditional Japanese herbal medicine (Kampo), has been used to treat symptoms of several diseases. In a recent clinical study, HKT was shown to be protective against the influenza virus infection. However, the underlying mechanism of the prophylactic effect is not clear. Mitochondrial and glycolytic pathways play important roles in cellular energy metabolism to maintain biological functions. These metabolic pathways are affected by the influenza virus infection. In this study, we examined the relationship between the preventive effects of HKT against the influenza virus infection and cellular energy metabolism in mitochondria and glycolysis using Madin-Darby canine kidney cells and influenza A/PR/8/34 (H1N1) virus (IAV). Methods: Mitochondrial and glycolytic metabolic pathways were evaluated on the basis of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), respectively, using the XF24 Extracellular Analyzer. Results: The OCR/ECAR ratio in IAV-infected cells was lower than that in control cells. Cells that were treated with HKT before IAV infection showed a metabolic pattern similar to that in the control cells (increase in both OCR and ECAR). Conclusions: Our results suggest that HKT not only activates both mitochondrial and glycolytic energy metabolism in IAV-infected cells but also helps maintain metabolic homeostasis similar to that in noninfected cells.

Original languageEnglish
Pages (from-to)240-249
Number of pages10
JournalPharmacology
DOIs
Publication statusAccepted/In press - 2017 Feb 2

Fingerprint

Herbal Medicine
Influenza A virus
Virus Diseases
Homeostasis
Orthomyxoviridae
Oxygen Consumption
Energy Metabolism
Metabolic Networks and Pathways
Kampo Medicine
H1N1 Subtype Influenza A Virus
Madin Darby Canine Kidney Cells
Glycolysis
Human Influenza
bu-zhong-yi-qi-tang
Mitochondria
Infection

Keywords

  • Cellular energy metabolism
  • Glycolysis
  • Herbal medicine
  • Hochuekkito
  • Influenza virus
  • Mitochondria
  • Preventive medicine

ASJC Scopus subject areas

  • Pharmacology

Cite this

Hochuekkito, a Japanese Herbal Medicine, Restores Metabolic Homeostasis between Mitochondrial and Glycolytic Pathways Impaired by Influenza A Virus Infection. / Takanashi, Keita; Dan, Katsuaki; Kanzaki, Sho; Hasegawa, Hideki; Watanabe, Kenji; Ogawa, Kaoru.

In: Pharmacology, 02.02.2017, p. 240-249.

Research output: Contribution to journalArticle

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abstract = "Background: Hochuekkito (HKT), a traditional Japanese herbal medicine (Kampo), has been used to treat symptoms of several diseases. In a recent clinical study, HKT was shown to be protective against the influenza virus infection. However, the underlying mechanism of the prophylactic effect is not clear. Mitochondrial and glycolytic pathways play important roles in cellular energy metabolism to maintain biological functions. These metabolic pathways are affected by the influenza virus infection. In this study, we examined the relationship between the preventive effects of HKT against the influenza virus infection and cellular energy metabolism in mitochondria and glycolysis using Madin-Darby canine kidney cells and influenza A/PR/8/34 (H1N1) virus (IAV). Methods: Mitochondrial and glycolytic metabolic pathways were evaluated on the basis of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), respectively, using the XF24 Extracellular Analyzer. Results: The OCR/ECAR ratio in IAV-infected cells was lower than that in control cells. Cells that were treated with HKT before IAV infection showed a metabolic pattern similar to that in the control cells (increase in both OCR and ECAR). Conclusions: Our results suggest that HKT not only activates both mitochondrial and glycolytic energy metabolism in IAV-infected cells but also helps maintain metabolic homeostasis similar to that in noninfected cells.",
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