Homeodomain transcription factor Meis1 is a critical regulator of adult bone marrow hematopoiesis

Reina Ariki, Satoru Morikawa, Yo Mabuchi, Sadafumi Suzuki, Mayuka Nakatake, Kentaro Yoshioka, Shinya Hidano, Hiromitsu Nakauchi, Yumi Matsuzaki, Takuro Nakamura, Ryo Goitsuka

Research output: Contribution to journalArticle

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Abstract

Hematopoietic stem cells in the bone marrow have the capacity to both self-renew and to generate all cells of the hematopoietic system. The balance of these two activities is controlled by hematopoietic stem cell-intrinsic regulatory mechanisms as well as extrinsic signals from the microenvironment. Here we demonstrate that Meis1, a TALE family homeodomain transcription factor involved in numerous embryonic developmental processes, is selectively expressed in hematopoietic stem/progenitor cells. Conditional Meis1 knockout in adult hematopoietic cells resulted in a significant reduction in the hematopoietic stem/progenitor cells. Suppression of hematopoiesis by Meis1 deletion appears to be caused by impaired self-renewal activity and reduced cellular quiescence of hematopoietic stem/progenitor cells in a cell autonomous manner, resulting in stem cell exhaustion and defective long-term hematopoiesis. Meis1 deficiency down-regulated a subset of Pbx1-dependent hematopoietic stem cell signature genes, suggesting a functional link between them in the maintenance of hematopoietic stem/progenitor cells. These results show the importance of Meis1 in adult hematopoiesis.

Original languageEnglish
Article numbere87646
JournalPLoS One
Volume9
Issue number2
DOIs
Publication statusPublished - 2014 Feb 3

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hematopoiesis
Hematopoiesis
Hematopoietic Stem Cells
Stem cells
bone marrow
stem cells
Bone
Transcription Factors
transcription factors
Bone Marrow
Hematopoietic System
cells
Genes
Stem Cells
Maintenance

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ariki, R., Morikawa, S., Mabuchi, Y., Suzuki, S., Nakatake, M., Yoshioka, K., ... Goitsuka, R. (2014). Homeodomain transcription factor Meis1 is a critical regulator of adult bone marrow hematopoiesis. PLoS One, 9(2), [e87646]. https://doi.org/10.1371/journal.pone.0087646

Homeodomain transcription factor Meis1 is a critical regulator of adult bone marrow hematopoiesis. / Ariki, Reina; Morikawa, Satoru; Mabuchi, Yo; Suzuki, Sadafumi; Nakatake, Mayuka; Yoshioka, Kentaro; Hidano, Shinya; Nakauchi, Hiromitsu; Matsuzaki, Yumi; Nakamura, Takuro; Goitsuka, Ryo.

In: PLoS One, Vol. 9, No. 2, e87646, 03.02.2014.

Research output: Contribution to journalArticle

Ariki, R, Morikawa, S, Mabuchi, Y, Suzuki, S, Nakatake, M, Yoshioka, K, Hidano, S, Nakauchi, H, Matsuzaki, Y, Nakamura, T & Goitsuka, R 2014, 'Homeodomain transcription factor Meis1 is a critical regulator of adult bone marrow hematopoiesis', PLoS One, vol. 9, no. 2, e87646. https://doi.org/10.1371/journal.pone.0087646
Ariki, Reina ; Morikawa, Satoru ; Mabuchi, Yo ; Suzuki, Sadafumi ; Nakatake, Mayuka ; Yoshioka, Kentaro ; Hidano, Shinya ; Nakauchi, Hiromitsu ; Matsuzaki, Yumi ; Nakamura, Takuro ; Goitsuka, Ryo. / Homeodomain transcription factor Meis1 is a critical regulator of adult bone marrow hematopoiesis. In: PLoS One. 2014 ; Vol. 9, No. 2.
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