Homeostatic pruning and activity of epidermal nerves are dysregulated in barrier-impaired skin during chronic itch development

Sonoko Takahashi, Azusa Ishida, Akiharu Kubo, Hiroshi Kawasaki, Sotaro Ochiai, Manabu Nakayama, Haruhiko Koseki, Masayuki Amagai, Takaharu Okada

Research output: Contribution to journalArticle

Abstract

The epidermal barrier is thought to protect sensory nerves from overexposure to environmental stimuli, and barrier impairment leads to pathological conditions associated with itch, such as atopic dermatitis (AD). However, it is not known how the epidermal barrier continuously protects nerves for the sensory homeostasis during turnover of the epidermis. Here we show that epidermal nerves are contained underneath keratinocyte tight junctions (TJs) in normal human and mouse skin, but not in human AD samples or mouse models of chronic itch caused by epidermal barrier impairment. By intravital imaging of the mouse skin, we found that epidermal nerve endings were frequently extended and retracted, and occasionally underwent local pruning. Importantly, the epidermal nerve pruning took place rapidly at intersections with newly forming TJs in the normal skin, whereas this process was disturbed during chronic itch development. Furthermore, aberrant Ca2+ increases in epidermal nerves were induced in association with the disturbed pruning. Finally, TRPA1 inhibition suppressed aberrant Ca2+ increases in epidermal nerves and itch. These results suggest that epidermal nerve endings are pruned through interactions with keratinocytes to stay below the TJ barrier, and that disruption of this mechanism may lead to aberrant activation of epidermal nerves and pathological itch.

Original languageEnglish
Article number8625
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

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Tight Junctions
Nerve Endings
Atopic Dermatitis
Keratinocytes
Skin
Epidermis
Homeostasis

ASJC Scopus subject areas

  • General

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Homeostatic pruning and activity of epidermal nerves are dysregulated in barrier-impaired skin during chronic itch development. / Takahashi, Sonoko; Ishida, Azusa; Kubo, Akiharu; Kawasaki, Hiroshi; Ochiai, Sotaro; Nakayama, Manabu; Koseki, Haruhiko; Amagai, Masayuki; Okada, Takaharu.

In: Scientific reports, Vol. 9, No. 1, 8625, 01.12.2019.

Research output: Contribution to journalArticle

Takahashi, Sonoko ; Ishida, Azusa ; Kubo, Akiharu ; Kawasaki, Hiroshi ; Ochiai, Sotaro ; Nakayama, Manabu ; Koseki, Haruhiko ; Amagai, Masayuki ; Okada, Takaharu. / Homeostatic pruning and activity of epidermal nerves are dysregulated in barrier-impaired skin during chronic itch development. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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