Homologous Recombination-Enhancing Factors Identified by Comparative Transcriptomic Analyses of Pluripotent Stem Cell of Human and Common Marmoset

Sho Yoshimatsu, Mayutaka Nakajima, Emi Qian, Tsukasa Sanosaka, Tsukika Sato, Hideyuki Okano

Research output: Contribution to journalArticlepeer-review

Abstract

A previous study assessing the efficiency of the genome editing technology CRISPR-Cas9 for knock-in gene targeting in common marmoset (marmoset; Callithrix jacchus) embryonic stem cells (ESCs) unexpectedly identified innately enhanced homologous recombination activity in marmoset ESCs. Here, we compared gene expression in marmoset and human pluripotent stem cells using transcriptomic and quantitative PCR analyses and found that five HR-related genes (BRCA1, BRCA2, RAD51C, RAD51D, and RAD51) were upregulated in marmoset cells. A total of four of these upregulated genes enhanced HR efficiency with CRISPR-Cas9 in human pluripotent stem cells. Thus, the present study provides a novel insight into species-specific mechanisms for the choice of DNA repair pathways.

Original languageEnglish
JournalCells
Volume11
Issue number3
DOIs
Publication statusPublished - 2022 Jan 21

Keywords

  • common marmoset
  • homologous recombination
  • pluripotent stem cells

ASJC Scopus subject areas

  • Medicine(all)

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