Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine

Narudo Kawai, Yosuke Ogura, Tetsuro Ikuta, Hidetoshi Saiga, Mayuko Hamada, Tetsushi Sakuma, Takashi Yamamoto, Nori Satoh, Yasunori Sasakura

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hox cluster genes play crucial roles in development of the metazoan antero-posterior axis. Functions of Hox genes in patterning the central nervous system and limb buds are well known. They are also expressed in chordate endodermal tissues, where their roles in endodermal development are still poorly understood. In the invertebrate chordate, Ciona intestinalis, endodermal tissues are in a premature state during the larval stage, and they differentiate into the digestive tract during metamorphosis. In this study, we showed that disruption of a Hox gene, Ci-Hox10, prevented intestinal formation. Ci-Hox10-knock-down larvae displayed defective migration of endodermal strand cells. Formation of a protrusion, which is important for cell migration, was disrupted in these cells. The collagen type IX gene is a downstream target of Ci-Hox10, and is negatively regulated by Ci-Hox10 and a matrix metalloproteinase ortholog, prior to endodermal cell migration. Inhibition of this regulation prevented cellular migration. These results suggest that Ci-Hox10 regulates endodermal strand cell migration by forming a protrusion and by reconstructing the extracellular matrix.

Original languageEnglish
Pages (from-to)43-56
Number of pages14
JournalDevelopmental Biology
Volume403
Issue number1
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

Urochordata
Homeobox Genes
Intestines
Cell Movement
Nonvertebrate Chordata
Collagen Type IX
Ciona intestinalis
Chordata
Limb Buds
Matrix Metalloproteinases
Extracellular Matrix
Larva
Gastrointestinal Tract
Central Nervous System
Genes

Keywords

  • Cell migration
  • Collagen type IX
  • Extracellular matrix
  • Hox
  • Intestine
  • Protrusion

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Kawai, N., Ogura, Y., Ikuta, T., Saiga, H., Hamada, M., Sakuma, T., ... Sasakura, Y. (2015). Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine. Developmental Biology, 403(1), 43-56. https://doi.org/10.1016/j.ydbio.2015.03.018

Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine. / Kawai, Narudo; Ogura, Yosuke; Ikuta, Tetsuro; Saiga, Hidetoshi; Hamada, Mayuko; Sakuma, Tetsushi; Yamamoto, Takashi; Satoh, Nori; Sasakura, Yasunori.

In: Developmental Biology, Vol. 403, No. 1, 01.07.2015, p. 43-56.

Research output: Contribution to journalArticle

Kawai, N, Ogura, Y, Ikuta, T, Saiga, H, Hamada, M, Sakuma, T, Yamamoto, T, Satoh, N & Sasakura, Y 2015, 'Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine', Developmental Biology, vol. 403, no. 1, pp. 43-56. https://doi.org/10.1016/j.ydbio.2015.03.018
Kawai, Narudo ; Ogura, Yosuke ; Ikuta, Tetsuro ; Saiga, Hidetoshi ; Hamada, Mayuko ; Sakuma, Tetsushi ; Yamamoto, Takashi ; Satoh, Nori ; Sasakura, Yasunori. / Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine. In: Developmental Biology. 2015 ; Vol. 403, No. 1. pp. 43-56.
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