HpDTC1, a Stress-Inducible Bifunctional Diterpene Cyclase Involved in Momilactone Biosynthesis, Functions in Chemical Defence in the Moss Hypnum plumaeforme

Kazunori Okada, Hiroshi Kawaide, Koji Miyamoto, Sho Miyazaki, Ryosuke Kainuma, Honoka Kimura, Kaoru Fujiwara, Masahiro Natsume, Hideaki Nojiri, Masatoshi Nakajima, Hisakazu Yamane, Yuki Hatano, Hiroshi Nozaki, Ken Ichiro Hayashi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Momilactones, which are diterpenoid phytoalexins with antimicrobial and allelopathic functions, have been found only in rice and the moss Hypnum plumaeforme. Although these two evolutionarily distinct plant species are thought to produce momilactones as a chemical defence, the momilactone biosynthetic pathway in H. plumaeforme has been unclear. Here, we identified a gene encoding syn-pimara-7,15-diene synthase (HpDTC1) responsible for the first step of momilactone biosynthesis in the moss. HpDTC1 is a bifunctional diterpene cyclase that catalyses a two-step cyclization reaction of geranylgeranyl diphosphate to syn-pimara-7,15-diene. HpDTC1 transcription was up-regulated in response to abiotic and biotic stress treatments. HpDTC1 promoter-GUS analysis in transgenic Physcomitrella patens showed similar transcriptional responses as H. plumaeforme to the stresses, suggesting that a common response system to stress exists in mosses. Jasmonic acid (JA), a potent signalling molecule for inducing plant defences, could not activate HpDTC1 expression. In contrast, 12-oxo-phytodienoic acid, an oxylipin precursor of JA in vascular plants, enhanced HpDTC1 expression and momilactone accumulation, implying that as-yet-unknown oxylipins could regulate momilactone biosynthesis in H. plumaeforme. These results demonstrate the existence of an evolutionarily conserved chemical defence system utilizing momilactones and suggest the molecular basis of the regulation for inductive production of momilactones in H. plumaeforme.

Original languageEnglish
Article number25316
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 May 3
Externally publishedYes

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Bryophyta
Diterpenes
Oxylipins
Bryopsida
Biosynthetic Pathways
Cyclization
Blood Vessels
Genes
pimara-7,15-diene
jasmonic acid

ASJC Scopus subject areas

  • General

Cite this

HpDTC1, a Stress-Inducible Bifunctional Diterpene Cyclase Involved in Momilactone Biosynthesis, Functions in Chemical Defence in the Moss Hypnum plumaeforme. / Okada, Kazunori; Kawaide, Hiroshi; Miyamoto, Koji; Miyazaki, Sho; Kainuma, Ryosuke; Kimura, Honoka; Fujiwara, Kaoru; Natsume, Masahiro; Nojiri, Hideaki; Nakajima, Masatoshi; Yamane, Hisakazu; Hatano, Yuki; Nozaki, Hiroshi; Hayashi, Ken Ichiro.

In: Scientific Reports, Vol. 6, 25316, 03.05.2016.

Research output: Contribution to journalArticle

Okada, K, Kawaide, H, Miyamoto, K, Miyazaki, S, Kainuma, R, Kimura, H, Fujiwara, K, Natsume, M, Nojiri, H, Nakajima, M, Yamane, H, Hatano, Y, Nozaki, H & Hayashi, KI 2016, 'HpDTC1, a Stress-Inducible Bifunctional Diterpene Cyclase Involved in Momilactone Biosynthesis, Functions in Chemical Defence in the Moss Hypnum plumaeforme', Scientific Reports, vol. 6, 25316. https://doi.org/10.1038/srep25316
Okada, Kazunori ; Kawaide, Hiroshi ; Miyamoto, Koji ; Miyazaki, Sho ; Kainuma, Ryosuke ; Kimura, Honoka ; Fujiwara, Kaoru ; Natsume, Masahiro ; Nojiri, Hideaki ; Nakajima, Masatoshi ; Yamane, Hisakazu ; Hatano, Yuki ; Nozaki, Hiroshi ; Hayashi, Ken Ichiro. / HpDTC1, a Stress-Inducible Bifunctional Diterpene Cyclase Involved in Momilactone Biosynthesis, Functions in Chemical Defence in the Moss Hypnum plumaeforme. In: Scientific Reports. 2016 ; Vol. 6.
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abstract = "Momilactones, which are diterpenoid phytoalexins with antimicrobial and allelopathic functions, have been found only in rice and the moss Hypnum plumaeforme. Although these two evolutionarily distinct plant species are thought to produce momilactones as a chemical defence, the momilactone biosynthetic pathway in H. plumaeforme has been unclear. Here, we identified a gene encoding syn-pimara-7,15-diene synthase (HpDTC1) responsible for the first step of momilactone biosynthesis in the moss. HpDTC1 is a bifunctional diterpene cyclase that catalyses a two-step cyclization reaction of geranylgeranyl diphosphate to syn-pimara-7,15-diene. HpDTC1 transcription was up-regulated in response to abiotic and biotic stress treatments. HpDTC1 promoter-GUS analysis in transgenic Physcomitrella patens showed similar transcriptional responses as H. plumaeforme to the stresses, suggesting that a common response system to stress exists in mosses. Jasmonic acid (JA), a potent signalling molecule for inducing plant defences, could not activate HpDTC1 expression. In contrast, 12-oxo-phytodienoic acid, an oxylipin precursor of JA in vascular plants, enhanced HpDTC1 expression and momilactone accumulation, implying that as-yet-unknown oxylipins could regulate momilactone biosynthesis in H. plumaeforme. These results demonstrate the existence of an evolutionarily conserved chemical defence system utilizing momilactones and suggest the molecular basis of the regulation for inductive production of momilactones in H. plumaeforme.",
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AU - Nojiri, Hideaki

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