Hsa-miR-346 is a potential serum biomarker of Mycobacterium avium complex pulmonary disease activity

Tomoyasu Nishimura, Eiko Tamizu, Shunsuke Uno, Yoshifumi Uwamino, Hiroshi Fujiwara, Kazumi Nishio, Yasushi Nakano, Hirofumi Shiono, Ho Namkoong, Yoshihiko Hoshino, Satoshi Iwata, Naoki Hasegawa

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Abstract

MicroRNA (miRNA) has been recently recognized as a biomarker of various diseases; however, there are no known miRNAs associated with Mycobacterium avium complex (MAC) pulmonary disease. In addition, there are no known biomarkers to precisely reflect disease activity after the diagnosis of MAC pulmonary disease. Thus, we sought to identify a miRNA which is a candidate biomarker of MAC pulmonary disease activity. Serum hsa-miR-346 concentrations of 16 patients with M. avium pulmonary disease were significantly higher than those of 16 healthy controls (p = 0.047). The secretion of hsa-miR-346 increased in a multiplicity of infection-dependent manner in M. avium-infected macrophages. Serum hsa-miR-346 levels of 5 patients with bacterial conversion at the end of follow-up were significantly lower than those at the beginning of the follow-up (p = 0.043). In addition, the longitudinal change in serum hsa-miR-346 concentration correlated with bacterial load in 2 patients with M. avium pulmonary disease. Based on our results, it is supposed that MAC-infected macrophages in pulmonary lesions produce hsa-miR-346, which is then secreted into the bloodstream. The magnitude of this process could be quantitatively controlled by the bacterial load, suggesting that serum hsa-miR-346 is a potentially useful biomarker of MAC pulmonary disease activity.

Original languageEnglish
JournalJournal of Infection and Chemotherapy
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Mycobacterium avium Complex
Lung Diseases
Biomarkers
Mycobacterium avium
Serum
MicroRNAs
Bacterial Load
Alveolar Macrophages
human MIRN346 microRNA
Macrophages
Infection

Keywords

  • Macrophage
  • MicroRNA
  • Mycobacterium avium complex

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Hsa-miR-346 is a potential serum biomarker of Mycobacterium avium complex pulmonary disease activity. / Nishimura, Tomoyasu; Tamizu, Eiko; Uno, Shunsuke; Uwamino, Yoshifumi; Fujiwara, Hiroshi; Nishio, Kazumi; Nakano, Yasushi; Shiono, Hirofumi; Namkoong, Ho; Hoshino, Yoshihiko; Iwata, Satoshi; Hasegawa, Naoki.

In: Journal of Infection and Chemotherapy, 2017.

Research output: Contribution to journalArticle

Nishimura, Tomoyasu ; Tamizu, Eiko ; Uno, Shunsuke ; Uwamino, Yoshifumi ; Fujiwara, Hiroshi ; Nishio, Kazumi ; Nakano, Yasushi ; Shiono, Hirofumi ; Namkoong, Ho ; Hoshino, Yoshihiko ; Iwata, Satoshi ; Hasegawa, Naoki. / Hsa-miR-346 is a potential serum biomarker of Mycobacterium avium complex pulmonary disease activity. In: Journal of Infection and Chemotherapy. 2017.
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abstract = "MicroRNA (miRNA) has been recently recognized as a biomarker of various diseases; however, there are no known miRNAs associated with Mycobacterium avium complex (MAC) pulmonary disease. In addition, there are no known biomarkers to precisely reflect disease activity after the diagnosis of MAC pulmonary disease. Thus, we sought to identify a miRNA which is a candidate biomarker of MAC pulmonary disease activity. Serum hsa-miR-346 concentrations of 16 patients with M. avium pulmonary disease were significantly higher than those of 16 healthy controls (p = 0.047). The secretion of hsa-miR-346 increased in a multiplicity of infection-dependent manner in M. avium-infected macrophages. Serum hsa-miR-346 levels of 5 patients with bacterial conversion at the end of follow-up were significantly lower than those at the beginning of the follow-up (p = 0.043). In addition, the longitudinal change in serum hsa-miR-346 concentration correlated with bacterial load in 2 patients with M. avium pulmonary disease. Based on our results, it is supposed that MAC-infected macrophages in pulmonary lesions produce hsa-miR-346, which is then secreted into the bloodstream. The magnitude of this process could be quantitatively controlled by the bacterial load, suggesting that serum hsa-miR-346 is a potentially useful biomarker of MAC pulmonary disease activity.",
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AU - Nishimura, Tomoyasu

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AU - Uno, Shunsuke

AU - Uwamino, Yoshifumi

AU - Fujiwara, Hiroshi

AU - Nishio, Kazumi

AU - Nakano, Yasushi

AU - Shiono, Hirofumi

AU - Namkoong, Ho

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AU - Iwata, Satoshi

AU - Hasegawa, Naoki

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AB - MicroRNA (miRNA) has been recently recognized as a biomarker of various diseases; however, there are no known miRNAs associated with Mycobacterium avium complex (MAC) pulmonary disease. In addition, there are no known biomarkers to precisely reflect disease activity after the diagnosis of MAC pulmonary disease. Thus, we sought to identify a miRNA which is a candidate biomarker of MAC pulmonary disease activity. Serum hsa-miR-346 concentrations of 16 patients with M. avium pulmonary disease were significantly higher than those of 16 healthy controls (p = 0.047). The secretion of hsa-miR-346 increased in a multiplicity of infection-dependent manner in M. avium-infected macrophages. Serum hsa-miR-346 levels of 5 patients with bacterial conversion at the end of follow-up were significantly lower than those at the beginning of the follow-up (p = 0.043). In addition, the longitudinal change in serum hsa-miR-346 concentration correlated with bacterial load in 2 patients with M. avium pulmonary disease. Based on our results, it is supposed that MAC-infected macrophages in pulmonary lesions produce hsa-miR-346, which is then secreted into the bloodstream. The magnitude of this process could be quantitatively controlled by the bacterial load, suggesting that serum hsa-miR-346 is a potentially useful biomarker of MAC pulmonary disease activity.

KW - Macrophage

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KW - Mycobacterium avium complex

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