Hsp70-mediated protein refolding in the E-Cell

Bin Hu, Matthias P. Mayer, Masaru Tomita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we used E-CELL, a software package aiming at large-scale modeling with full object-oriented modeling support, to analyze the 70kDa heat shock protein (Hsp70) chaperone mediated protein folding. We analyzed the kinetic characteristics of this chaperone system during folding of an unfolded protein using computer simulations. Our simulation results are consistent with laboratory experiments reported and support the kinetic partitioning hypothesis. Our model suggests that although the DnaK chaperone system is robust in assisting protein folding, this robustness is limited by the availability of ATP. Based on this model, we also discusse why object-oriented modeling is needed for reducing the complexity in large-scale biochemical models.

Original languageEnglish
Title of host publicationWMSCI 2005 - The 9th World Multi-Conference on Systemics, Cybernetics and Informatics, Proceedings
Pages377-382
Number of pages6
Publication statusPublished - 2005 Dec 1
Event9th World Multi-Conference on Systemics, Cybernetics and Informatics, WMSCI 2005 - Orlando, FL, United States
Duration: 2005 Jul 102005 Jul 13

Publication series

NameWMSCI 2005 - The 9th World Multi-Conference on Systemics, Cybernetics and Informatics, Proceedings
Volume8

Other

Other9th World Multi-Conference on Systemics, Cybernetics and Informatics, WMSCI 2005
CountryUnited States
CityOrlando, FL
Period05/7/1005/7/13

    Fingerprint

Keywords

  • ATP
  • Hsp70
  • Kinetic
  • Simulation and E-CELL

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Networks and Communications
  • Information Systems

Cite this

Hu, B., Mayer, M. P., & Tomita, M. (2005). Hsp70-mediated protein refolding in the E-Cell. In WMSCI 2005 - The 9th World Multi-Conference on Systemics, Cybernetics and Informatics, Proceedings (pp. 377-382). (WMSCI 2005 - The 9th World Multi-Conference on Systemics, Cybernetics and Informatics, Proceedings; Vol. 8).