Abstract
We investigated the transport of salicylic acid and L-lactic acid across the placenta using the human trophoblast cell line BeWo. We performed uptake experiments and measured the change in intracellular pH (pHi). The uptakes of [14C] salicylic acid and L-[14C] lactic acid were temperature- and extracellular pH-dependent and saturable at higher concentrations. Both uptakes were also reduced by FCCP, nigericin, and NaN3. Various nonsteroidal anti-inflammatory drugs (NSAIDs) strongly inhibited the uptake of L-[14C] lactic acid. Salicylic acid and ibuprofen noncompetitively inhibited the uptake of L-[14C] lactic acid. α-Cyano-4-hydroxycinnamate (CHC), a monocarboxylate transporter inhibitor, suppressed the uptake of L-[14C] lactic acid but not that of [14C] salicylic acid. CHC also suppressed the decrease of pHi induced by L-lactic acid but had little effect on that induced by salicylic acid or diclofenac. These results suggest that NSAIDs are potent inhibitors of lactate transporters, although they are transported mainly by a transport system distinct from that for L-lactic acid.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Cell Physiology |
| Volume | 282 |
| Issue number | 5 51-5 |
| Publication status | Published - 2002 |
| Externally published | Yes |
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Keywords
- α-cyano-4-hydroxycinnamate
- Blood-placental barrier
- Intracellular pH
- L-lactic acid
- Monocarboxylate transporter
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
Cite this
H+-linked transport of salicylic acid, an NSAID, in the human trophoblast cell line BeWo. / Emoto, Akiko; Ushigome, Fumihiko; Koyabu, Noriko; Kajiya, Hiroshi; Okabe, Koji; Satoh, Shoji; Tsukimori, Kiyomi; Nakano, Hitoo; Ohtani, Hisakazu; Sawada, Yasufumi.
In: American Journal of Physiology - Cell Physiology, Vol. 282, No. 5 51-5, 2002.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - H+-linked transport of salicylic acid, an NSAID, in the human trophoblast cell line BeWo
AU - Emoto, Akiko
AU - Ushigome, Fumihiko
AU - Koyabu, Noriko
AU - Kajiya, Hiroshi
AU - Okabe, Koji
AU - Satoh, Shoji
AU - Tsukimori, Kiyomi
AU - Nakano, Hitoo
AU - Ohtani, Hisakazu
AU - Sawada, Yasufumi
PY - 2002
Y1 - 2002
N2 - We investigated the transport of salicylic acid and L-lactic acid across the placenta using the human trophoblast cell line BeWo. We performed uptake experiments and measured the change in intracellular pH (pHi). The uptakes of [14C] salicylic acid and L-[14C] lactic acid were temperature- and extracellular pH-dependent and saturable at higher concentrations. Both uptakes were also reduced by FCCP, nigericin, and NaN3. Various nonsteroidal anti-inflammatory drugs (NSAIDs) strongly inhibited the uptake of L-[14C] lactic acid. Salicylic acid and ibuprofen noncompetitively inhibited the uptake of L-[14C] lactic acid. α-Cyano-4-hydroxycinnamate (CHC), a monocarboxylate transporter inhibitor, suppressed the uptake of L-[14C] lactic acid but not that of [14C] salicylic acid. CHC also suppressed the decrease of pHi induced by L-lactic acid but had little effect on that induced by salicylic acid or diclofenac. These results suggest that NSAIDs are potent inhibitors of lactate transporters, although they are transported mainly by a transport system distinct from that for L-lactic acid.
AB - We investigated the transport of salicylic acid and L-lactic acid across the placenta using the human trophoblast cell line BeWo. We performed uptake experiments and measured the change in intracellular pH (pHi). The uptakes of [14C] salicylic acid and L-[14C] lactic acid were temperature- and extracellular pH-dependent and saturable at higher concentrations. Both uptakes were also reduced by FCCP, nigericin, and NaN3. Various nonsteroidal anti-inflammatory drugs (NSAIDs) strongly inhibited the uptake of L-[14C] lactic acid. Salicylic acid and ibuprofen noncompetitively inhibited the uptake of L-[14C] lactic acid. α-Cyano-4-hydroxycinnamate (CHC), a monocarboxylate transporter inhibitor, suppressed the uptake of L-[14C] lactic acid but not that of [14C] salicylic acid. CHC also suppressed the decrease of pHi induced by L-lactic acid but had little effect on that induced by salicylic acid or diclofenac. These results suggest that NSAIDs are potent inhibitors of lactate transporters, although they are transported mainly by a transport system distinct from that for L-lactic acid.
KW - α-cyano-4-hydroxycinnamate
KW - Blood-placental barrier
KW - Intracellular pH
KW - L-lactic acid
KW - Monocarboxylate transporter
UR - http://www.scopus.com/inward/record.url?scp=17944402277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17944402277&partnerID=8YFLogxK
M3 - Article
VL - 282
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
SN - 0363-6143
IS - 5 51-5
ER -