HTLV-1 p27(rex) stabilizes human interleukin-2 receptor α chain mRNA

H. Kanamori, N. Suzuki, H. Siomi, T. Nosaka, A. Sato, H. Sabe, M. Hatanaka, T. Honjo

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Expression of the pX gene products (p40(tax), p27(rex) and p21(X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces expression of the interleukin-2 receptor α chain (IL-2Rα) on infected T cells. Comparison of IL-2Rα promoter activities has revealed that the transcriptional activation of the promoter alone cannot explain the large numbers of IL-2Rα expressed on HTLV-1 infected cells. We found that the rates of the IL-2Rα mRNA degradation were greatly reduced in pX-positive cells as compared with pX-negative cells. Simultaneous transfection of the expression vector plasmid containing IL-2Rα cDNA and similar plasmids containing various pX sequences showed that p27(rex) elongated the half life of IL-2Rα mRNA. As p27(rex) did not affect the transport of the IL-2Rα mRNA from nucleus to cytoplasm, prolongation of the IL-2Rα mRNA half life by p27(rex) is ascribed to stabilization of the mRNA. Experiments using deletion mutants and chimeric constructs of the IL-2Rα cDNA demonstrated that the coding sequence but not the 5' or 3' untranslated region of the IL-2Rα mRNA sequence is responsible for its protection by p27(rex).

Original languageEnglish
Pages (from-to)4161-4166
Number of pages6
JournalEMBO Journal
Volume9
Issue number12
Publication statusPublished - 1990 Nov 29

    Fingerprint

Keywords

  • coding sequence
  • interleukin-2 receptor α chain
  • mRNA regulation
  • p27(rex)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Kanamori, H., Suzuki, N., Siomi, H., Nosaka, T., Sato, A., Sabe, H., Hatanaka, M., & Honjo, T. (1990). HTLV-1 p27(rex) stabilizes human interleukin-2 receptor α chain mRNA. EMBO Journal, 9(12), 4161-4166.