HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA

Hiroshi Kanamori, Noboru Suzuki, Haruhiko Siomi, Tetsuya Nosaka, Akihiko Sato, Hisataka Sabe, Masakazu Hatanaka, Tasuku Honjo

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Expression of the pX gene products (p40tax, p27rex and p21X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces expression of the interleukin-2 receptor a chain (IL-2Rα) on infected T cells. Comparison of IL-2Rα promoter activities has revealed that the transcriptional activation of the promoter alone cannot explain the large numbers of IL-2Rα expressed on HTLV-1 infected cells. We found that the rates of the IL-2Rα mRNA degradation were greatly reduced in pX-positive cells as compared with pX-negative cells. Simultaneous transfection of the expression vector plasmid containing IL-2Rα cDNA and similar plasmids containing various pX sequences showed that p27rex elongated the half life of IL-2Rα mRNA. As p27rex did not affect the transport of the IL-2Rα mRNA from nucleus to cytoplasm, prolongation of the IL-2Rα mRNA half life by p27rex is ascribed to stabilization of the mRNA. Experiments using deletion mutants and chimeric constructs of the IL-2Rα cDNA demonstrated that the coding sequence but not the 5′ or 3′ untranslated region of the IL-2Rα mRNA sequence is responsible for its protection by p27rex.

Original languageEnglish
Pages (from-to)4161-4166
Number of pages6
JournalEMBO Journal
Volume9
Issue number12
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Deltaretrovirus
T-cells
Interleukin-2 Receptors
Viruses
Messenger RNA
Half-Life
Plasmids
pX Genes
Complementary DNA
Adult T Cell Leukemia Lymphoma
5' Untranslated Regions
RNA Stability
3' Untranslated Regions
Transcriptional Activation
Transfection
Cytoplasm
Stabilization
Genes
Chemical activation
Cells

Keywords

  • Coding sequence
  • Interluekin-2 receptor α chain
  • Mrna regulation
  • P27

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Kanamori, H., Suzuki, N., Siomi, H., Nosaka, T., Sato, A., Sabe, H., ... Honjo, T. (1990). HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA. EMBO Journal, 9(12), 4161-4166.

HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA. / Kanamori, Hiroshi; Suzuki, Noboru; Siomi, Haruhiko; Nosaka, Tetsuya; Sato, Akihiko; Sabe, Hisataka; Hatanaka, Masakazu; Honjo, Tasuku.

In: EMBO Journal, Vol. 9, No. 12, 1990, p. 4161-4166.

Research output: Contribution to journalArticle

Kanamori, H, Suzuki, N, Siomi, H, Nosaka, T, Sato, A, Sabe, H, Hatanaka, M & Honjo, T 1990, 'HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA', EMBO Journal, vol. 9, no. 12, pp. 4161-4166.
Kanamori H, Suzuki N, Siomi H, Nosaka T, Sato A, Sabe H et al. HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA. EMBO Journal. 1990;9(12):4161-4166.
Kanamori, Hiroshi ; Suzuki, Noboru ; Siomi, Haruhiko ; Nosaka, Tetsuya ; Sato, Akihiko ; Sabe, Hisataka ; Hatanaka, Masakazu ; Honjo, Tasuku. / HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA. In: EMBO Journal. 1990 ; Vol. 9, No. 12. pp. 4161-4166.
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