Abstract
Expression of the pX gene products (p40tax, p27rex and p21X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces expression of the interleukin-2 receptor a chain (IL-2Rα) on infected T cells. Comparison of IL-2Rα promoter activities has revealed that the transcriptional activation of the promoter alone cannot explain the large numbers of IL-2Rα expressed on HTLV-1 infected cells. We found that the rates of the IL-2Rα mRNA degradation were greatly reduced in pX-positive cells as compared with pX-negative cells. Simultaneous transfection of the expression vector plasmid containing IL-2Rα cDNA and similar plasmids containing various pX sequences showed that p27rex elongated the half life of IL-2Rα mRNA. As p27rex did not affect the transport of the IL-2Rα mRNA from nucleus to cytoplasm, prolongation of the IL-2Rα mRNA half life by p27rex is ascribed to stabilization of the mRNA. Experiments using deletion mutants and chimeric constructs of the IL-2Rα cDNA demonstrated that the coding sequence but not the 5′ or 3′ untranslated region of the IL-2Rα mRNA sequence is responsible for its protection by p27rex.
| Original language | English |
|---|---|
| Pages (from-to) | 4161-4166 |
| Number of pages | 6 |
| Journal | EMBO Journal |
| Volume | 9 |
| Issue number | 12 |
| Publication status | Published - 1990 |
| Externally published | Yes |
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Keywords
- Coding sequence
- Interluekin-2 receptor α chain
- Mrna regulation
- P27
ASJC Scopus subject areas
- Genetics
- Cell Biology
Cite this
HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA. / Kanamori, Hiroshi; Suzuki, Noboru; Siomi, Haruhiko; Nosaka, Tetsuya; Sato, Akihiko; Sabe, Hisataka; Hatanaka, Masakazu; Honjo, Tasuku.
In: EMBO Journal, Vol. 9, No. 12, 1990, p. 4161-4166.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HTLV-1 p27rex stabilizes human interleukin-2 receptor α chain mRNA
AU - Kanamori, Hiroshi
AU - Suzuki, Noboru
AU - Siomi, Haruhiko
AU - Nosaka, Tetsuya
AU - Sato, Akihiko
AU - Sabe, Hisataka
AU - Hatanaka, Masakazu
AU - Honjo, Tasuku
PY - 1990
Y1 - 1990
N2 - Expression of the pX gene products (p40tax, p27rex and p21X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces expression of the interleukin-2 receptor a chain (IL-2Rα) on infected T cells. Comparison of IL-2Rα promoter activities has revealed that the transcriptional activation of the promoter alone cannot explain the large numbers of IL-2Rα expressed on HTLV-1 infected cells. We found that the rates of the IL-2Rα mRNA degradation were greatly reduced in pX-positive cells as compared with pX-negative cells. Simultaneous transfection of the expression vector plasmid containing IL-2Rα cDNA and similar plasmids containing various pX sequences showed that p27rex elongated the half life of IL-2Rα mRNA. As p27rex did not affect the transport of the IL-2Rα mRNA from nucleus to cytoplasm, prolongation of the IL-2Rα mRNA half life by p27rex is ascribed to stabilization of the mRNA. Experiments using deletion mutants and chimeric constructs of the IL-2Rα cDNA demonstrated that the coding sequence but not the 5′ or 3′ untranslated region of the IL-2Rα mRNA sequence is responsible for its protection by p27rex.
AB - Expression of the pX gene products (p40tax, p27rex and p21X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces expression of the interleukin-2 receptor a chain (IL-2Rα) on infected T cells. Comparison of IL-2Rα promoter activities has revealed that the transcriptional activation of the promoter alone cannot explain the large numbers of IL-2Rα expressed on HTLV-1 infected cells. We found that the rates of the IL-2Rα mRNA degradation were greatly reduced in pX-positive cells as compared with pX-negative cells. Simultaneous transfection of the expression vector plasmid containing IL-2Rα cDNA and similar plasmids containing various pX sequences showed that p27rex elongated the half life of IL-2Rα mRNA. As p27rex did not affect the transport of the IL-2Rα mRNA from nucleus to cytoplasm, prolongation of the IL-2Rα mRNA half life by p27rex is ascribed to stabilization of the mRNA. Experiments using deletion mutants and chimeric constructs of the IL-2Rα cDNA demonstrated that the coding sequence but not the 5′ or 3′ untranslated region of the IL-2Rα mRNA sequence is responsible for its protection by p27rex.
KW - Coding sequence
KW - Interluekin-2 receptor α chain
KW - Mrna regulation
KW - P27
UR - http://www.scopus.com/inward/record.url?scp=0025146986&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025146986&partnerID=8YFLogxK
M3 - Article
VL - 9
SP - 4161
EP - 4166
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 12
ER -