Human and mouse ISLR (immunoglobulin superfamily containing leucine-rich repeat) genes: Genomic structure and tissue expression

Akemi Nagasawa, Jun Kudo, Setsuko Noda, Yukihiko Mashima, Alan Wright, Yoshihisa Oguchi, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have previously reported a transcript of the novel gene for human immunoglobulin superfamily containing leucine-rich repeat (ISLR). By additional screening of a human retina cDNA library, we isolated another type of transcript with a 5' UTR different from that of the previously reported type. Genomic sequencing of the ISLR gene revealed that these two types of transcripts, ISLR-1 and ISLR-2, originated from the same gene but are composed of different first exons. Because the entire open reading frame is contained in the second exon, these two transcripts produce the same protein. Radiation hybrid mapping linked the ISLR gene to AFM248yh1, which is in the critical region of Bardet-Biedl syndrome type 4 (BBS4) on chromosome 15. Sequence analysis of the ISLR gene in five BBS4 patients, however, showed no mutations, although a few polymorphic changes were detected. Cloning of the mouse homolog of ISLR (Islr) revealed that the predicted protein consists of 428 amino acids, 86% of which are identical to those of ISLR. The Islr gene was expressed in various mouse tissues, including retina, in which Islr mRNA was detected in the ganglion cell layer, the inner nuclear layer, and the inner segment of the photoreceptor.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalGenomics
Volume61
Issue number1
DOIs
Publication statusPublished - 1999 Oct 1

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Leucine
Immunoglobulins
Genes
Retina
Exons
Radiation Hybrid Mapping
Chromosomes, Human, Pair 15
Immunoglobulin Genes
5' Untranslated Regions
Gene Library
Ganglia
Open Reading Frames
Sequence Analysis
Organism Cloning
Proteins
Amino Acids
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Genetics

Cite this

Human and mouse ISLR (immunoglobulin superfamily containing leucine-rich repeat) genes : Genomic structure and tissue expression. / Nagasawa, Akemi; Kudo, Jun; Noda, Setsuko; Mashima, Yukihiko; Wright, Alan; Oguchi, Yoshihisa; Shimizu, Nobuyoshi.

In: Genomics, Vol. 61, No. 1, 01.10.1999, p. 37-43.

Research output: Contribution to journalArticle

Nagasawa, A, Kudo, J, Noda, S, Mashima, Y, Wright, A, Oguchi, Y & Shimizu, N 1999, 'Human and mouse ISLR (immunoglobulin superfamily containing leucine-rich repeat) genes: Genomic structure and tissue expression', Genomics, vol. 61, no. 1, pp. 37-43. https://doi.org/10.1006/geno.1999.5934
Nagasawa, Akemi ; Kudo, Jun ; Noda, Setsuko ; Mashima, Yukihiko ; Wright, Alan ; Oguchi, Yoshihisa ; Shimizu, Nobuyoshi. / Human and mouse ISLR (immunoglobulin superfamily containing leucine-rich repeat) genes : Genomic structure and tissue expression. In: Genomics. 1999 ; Vol. 61, No. 1. pp. 37-43.
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