Human autoantibodies against HD1/plectin in paraneoplastic pemphigus

Charlotte Proby, Yoshiko Fujii, Katsushi Owaribe, Takeji Nishikawa, Masayuki Amagai

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease that occurs in association with underlying neoplasms. PNP patients develop characteristic autoantibodies directed against multiple antigens, mostly identified as members of the plakin family of cytoplasmic proteins (desmoplakin I and II, bullous pemphigoid antigen I, envoplakin, and periplakin). HD1/plectin, another member of the plakin family, has not previously been detected in the characteristic PNP antigen complex, which may relate to practical difficulties associated with its large size (molecular weight ≃ 500 kDa). In this study, a combination of immunoprecipitation and immunoblot is used to demonstrate that HD1/plectin is also recognized by sera from PNP patients. Thirteen of 16 PNP sera tested were positive for HD1/plectin compared with none of 43 control sera (11 pemphigus vulgaris, 11 pemphigus foliaceus, 11 bullous pemphigoid, and 10 normal individuals). Combined with our recent finding that desmoglein 3 and desmoglein 1 are cell surface target antigens in PNP, this demonstration of plectin/HD1 as another component of the antigen complex in PNP confirms that PNP is an autoimmune disease against desmoglein and plakin family molecules.

Original languageEnglish
Pages (from-to)153-156
Number of pages4
JournalJournal of Investigative Dermatology
Volume112
Issue number2
DOIs
Publication statusPublished - 1999

Fingerprint

Plectin
Pemphigus
Autoantibodies
Desmoplakins
Antigens
Desmogleins
Desmoglein 3
Desmoglein 1
Bullous Pemphigoid
Demonstrations
Molecular weight
Autoimmune Diseases
Molecules
Serum
Surface Antigens
Proteins
Immunoprecipitation

Keywords

  • Autoimmunity
  • Desmoglein
  • Plakins

ASJC Scopus subject areas

  • Dermatology

Cite this

Human autoantibodies against HD1/plectin in paraneoplastic pemphigus. / Proby, Charlotte; Fujii, Yoshiko; Owaribe, Katsushi; Nishikawa, Takeji; Amagai, Masayuki.

In: Journal of Investigative Dermatology, Vol. 112, No. 2, 1999, p. 153-156.

Research output: Contribution to journalArticle

Proby, Charlotte ; Fujii, Yoshiko ; Owaribe, Katsushi ; Nishikawa, Takeji ; Amagai, Masayuki. / Human autoantibodies against HD1/plectin in paraneoplastic pemphigus. In: Journal of Investigative Dermatology. 1999 ; Vol. 112, No. 2. pp. 153-156.
@article{bf29c9d146c246188f10e1f2a5e2081b,
title = "Human autoantibodies against HD1/plectin in paraneoplastic pemphigus",
abstract = "Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease that occurs in association with underlying neoplasms. PNP patients develop characteristic autoantibodies directed against multiple antigens, mostly identified as members of the plakin family of cytoplasmic proteins (desmoplakin I and II, bullous pemphigoid antigen I, envoplakin, and periplakin). HD1/plectin, another member of the plakin family, has not previously been detected in the characteristic PNP antigen complex, which may relate to practical difficulties associated with its large size (molecular weight ≃ 500 kDa). In this study, a combination of immunoprecipitation and immunoblot is used to demonstrate that HD1/plectin is also recognized by sera from PNP patients. Thirteen of 16 PNP sera tested were positive for HD1/plectin compared with none of 43 control sera (11 pemphigus vulgaris, 11 pemphigus foliaceus, 11 bullous pemphigoid, and 10 normal individuals). Combined with our recent finding that desmoglein 3 and desmoglein 1 are cell surface target antigens in PNP, this demonstration of plectin/HD1 as another component of the antigen complex in PNP confirms that PNP is an autoimmune disease against desmoglein and plakin family molecules.",
keywords = "Autoimmunity, Desmoglein, Plakins",
author = "Charlotte Proby and Yoshiko Fujii and Katsushi Owaribe and Takeji Nishikawa and Masayuki Amagai",
year = "1999",
doi = "10.1046/j.1523-1747.1999.00498.x",
language = "English",
volume = "112",
pages = "153--156",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Human autoantibodies against HD1/plectin in paraneoplastic pemphigus

AU - Proby, Charlotte

AU - Fujii, Yoshiko

AU - Owaribe, Katsushi

AU - Nishikawa, Takeji

AU - Amagai, Masayuki

PY - 1999

Y1 - 1999

N2 - Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease that occurs in association with underlying neoplasms. PNP patients develop characteristic autoantibodies directed against multiple antigens, mostly identified as members of the plakin family of cytoplasmic proteins (desmoplakin I and II, bullous pemphigoid antigen I, envoplakin, and periplakin). HD1/plectin, another member of the plakin family, has not previously been detected in the characteristic PNP antigen complex, which may relate to practical difficulties associated with its large size (molecular weight ≃ 500 kDa). In this study, a combination of immunoprecipitation and immunoblot is used to demonstrate that HD1/plectin is also recognized by sera from PNP patients. Thirteen of 16 PNP sera tested were positive for HD1/plectin compared with none of 43 control sera (11 pemphigus vulgaris, 11 pemphigus foliaceus, 11 bullous pemphigoid, and 10 normal individuals). Combined with our recent finding that desmoglein 3 and desmoglein 1 are cell surface target antigens in PNP, this demonstration of plectin/HD1 as another component of the antigen complex in PNP confirms that PNP is an autoimmune disease against desmoglein and plakin family molecules.

AB - Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease that occurs in association with underlying neoplasms. PNP patients develop characteristic autoantibodies directed against multiple antigens, mostly identified as members of the plakin family of cytoplasmic proteins (desmoplakin I and II, bullous pemphigoid antigen I, envoplakin, and periplakin). HD1/plectin, another member of the plakin family, has not previously been detected in the characteristic PNP antigen complex, which may relate to practical difficulties associated with its large size (molecular weight ≃ 500 kDa). In this study, a combination of immunoprecipitation and immunoblot is used to demonstrate that HD1/plectin is also recognized by sera from PNP patients. Thirteen of 16 PNP sera tested were positive for HD1/plectin compared with none of 43 control sera (11 pemphigus vulgaris, 11 pemphigus foliaceus, 11 bullous pemphigoid, and 10 normal individuals). Combined with our recent finding that desmoglein 3 and desmoglein 1 are cell surface target antigens in PNP, this demonstration of plectin/HD1 as another component of the antigen complex in PNP confirms that PNP is an autoimmune disease against desmoglein and plakin family molecules.

KW - Autoimmunity

KW - Desmoglein

KW - Plakins

UR - http://www.scopus.com/inward/record.url?scp=0032916947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032916947&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1747.1999.00498.x

DO - 10.1046/j.1523-1747.1999.00498.x

M3 - Article

C2 - 9989789

AN - SCOPUS:0032916947

VL - 112

SP - 153

EP - 156

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -