TY - JOUR
T1 - Human blood metabolite timetable indicates internal body time
AU - Kasukawa, Takeya
AU - Sugimoto, Masahiro
AU - Hida, Akiko
AU - Minami, Yoichi
AU - Mori, Masayo
AU - Honma, Sato
AU - Honma, Ken Ichi
AU - Mishima, Kazuo
AU - Soga, Tomoyoshi
AU - Ueda, Hiroki R.
N1 - Funding Information:
We thank Shinobu Abe and Yasuko Sakaguchi at the Institute for Advanced Biosciences (IAB) for metabolomic analysis; Masahiro Hayashi, Hiroshi Fujishima, and Tomoyo Hashiguchi at RIKEN Center for Developmental Biology (CDB) for handling samples; Hiroyuki Tamiya and Ryohei Narumi at RIKEN CDB for helpful discussion; Arthur Millius at RIKEN CDB for helpful discussion and for proofreading of the manuscript; and Takuro Ito at IAB, Keio University for providing materials used in the identification of oscillating metabolites. This research was supported by an intramural grant-in-aid from the RIKEN CDB (to H.R.U.), research funds from New Energy Developing Organization (to H.R.U.), research funds from the Yamagata Prefectural Government and the city of Tsuruoka (to M.S., M.M., and T.S.), a grant-in-aid for the Strategic Research Program for Brain Sciences from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to K.M.), an Intramural Research Grant (No. 23-3) for Neurological and Psychiatric Disorders of National Center of Neurology and Psychiatry, and a Grant-in Aid for Scientific Research (No. 21390335) from Japan Society for the Promotion of Science (to K.M. and A.H.).
Funding Information:
and T.S.), a grant-in-aid for the Strategic Research Program for Brain Sciences from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to K.M.), an Intramural Research Grant (No. 23-3) for Neurological and Psychiatric Disorders of National Center of Neurology and Psychiatry, and a Grant-in Aid for Scientific Research (No. 21390335) from Japan Society for the Promotion of Science (to K.M. and A.H.).
Funding Information:
ACKNOWLEDGMENTS. We thank Shinobu Abe and Yasuko Sakaguchi at the Institute for Advanced Biosciences (IAB) for metabolomic analysis; Masahiro Hayashi, Hiroshi Fujishima, and Tomoyo Hashiguchi at RIKEN Center for Developmental Biology (CDB) for handling samples; Hiroyuki Tamiya and Ryohei Narumi at RIKEN CDB for helpful discussion; Arthur Millius at RIKEN CDB for helpful discussion and for proofreading of the manuscript; and Takuro Ito at IAB, Keio University for providing materials used in the identification of oscillating metabolites. This research was supported by an intramural grant-in-aid from the RIKEN CDB (to H.R.U.), research funds from New Energy Developing Organization (to H.R.U.), research funds from the Yamagata Prefectural Government and the city of Tsuruoka (to M.S., M.M.,
Publisher Copyright:
© 2012, National Academy of Sciences. All rights reserved.
PY - 2012/9/11
Y1 - 2012/9/11
N2 - A convenient way to estimate internal body time (BT) is essential for chronotherapy and time-restricted feeding, both of which use body-time information to maximize potency and minimize toxicity during drug administration and feeding, respectively. Previously, we proposed a molecular timetable based on circadian-oscillating substances in multiple mouse organs or blood to estimate internal body time from samples taken at only a few time points. Here we applied this molecular-timetable concept to estimate and evaluate internal body time in humans. We constructed a 1.5-d reference timetable of oscillating metabolites in human blood samples with 2-h sampling frequency while simultaneously controlling for the confounding effects of activity level, light, temperature, sleep, and food intake. By using this metabolite timetable as a reference, we accurately determined internal body time within 3 h from just two anti-phase blood samples. Our minimally invasive, molecular-timetable method with human blood enables highly optimized and personalized medicine.
AB - A convenient way to estimate internal body time (BT) is essential for chronotherapy and time-restricted feeding, both of which use body-time information to maximize potency and minimize toxicity during drug administration and feeding, respectively. Previously, we proposed a molecular timetable based on circadian-oscillating substances in multiple mouse organs or blood to estimate internal body time from samples taken at only a few time points. Here we applied this molecular-timetable concept to estimate and evaluate internal body time in humans. We constructed a 1.5-d reference timetable of oscillating metabolites in human blood samples with 2-h sampling frequency while simultaneously controlling for the confounding effects of activity level, light, temperature, sleep, and food intake. By using this metabolite timetable as a reference, we accurately determined internal body time within 3 h from just two anti-phase blood samples. Our minimally invasive, molecular-timetable method with human blood enables highly optimized and personalized medicine.
KW - Circadian rhythm
KW - Diagnostic tool
KW - Liquid chromatography mass spectrometry
KW - Metabolomics
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U2 - 10.1073/pnas.1207768109
DO - 10.1073/pnas.1207768109
M3 - Article
C2 - 22927403
AN - SCOPUS:84871917034
VL - 109
SP - 15036
EP - 15041
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 37
ER -