Human bronchial smooth muscle cell proliferation via thromboxane A 2 receptor

Yusuke Suzuki, Koichiro Asano, Yoshiki Shiraishi, Tsuyoshi Oguma, Tetsuya Shiomi, Koichi Fukunaga, Takeshi Nakajima, Kyoko Niimi, Kazuhiro Yamaguchi, Akitoshi Ishizaka

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Abstract

Thromboxane A 2 receptor (TP) mediates bronchial smooth muscle cell (BSMC) contraction, airway hyperresponsiveness, and airway inflammation in patients with asthma. In the present study, a pathogenic role of TP activation in airway remodeling was examined using primary cultures of human BSMC. A TP agonist, I-BOP, concentration-dependently enhanced not only bromodeoxyuridine (BrdU) uptake but also cell proliferation of BSMC. A TP-selective antagonist, AA-2414, blocked the effects of I-BOP on both BrdU uptake and cell proliferation. I-BOP-induced BrdU uptake was significantly blocked by two non-selective tyrosine kinase inhibitors, genistein and herbimycin A, or a Src family tyrosine kinase inhibitor, PP2, but not by an inhibitor of epidermal growth factor (EGF) receptor-associated tyrosine kinase, AG1478. In conclusion, TP receptor activation causes DNA synthesis and cell proliferation of human BSMC by activating tyrosine kinases including Src, but not by EGF receptor transactivation.

Original languageEnglish
Pages (from-to)375-382
Number of pages8
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume71
Issue number6
DOIs
Publication statusPublished - 2004 Dec

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Suzuki, Y., Asano, K., Shiraishi, Y., Oguma, T., Shiomi, T., Fukunaga, K., Nakajima, T., Niimi, K., Yamaguchi, K., & Ishizaka, A. (2004). Human bronchial smooth muscle cell proliferation via thromboxane A 2 receptor. Prostaglandins Leukotrienes and Essential Fatty Acids, 71(6), 375-382. https://doi.org/10.1016/j.plefa.2004.07.004