Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation

Masataka Kuwana, Yuka Okazaki, Hiroaki Kodama, Keisuke Izumi, Hidekata Yasuoka, Yoko Ogawa, Yutaka Kawakami, Yasuo Ikeda

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Circulating CD14+ monocytes are precursors of phagocytes, such as macrophages and dendritic cells. Here we report primitive cells with a fibroblast-like morphology derived from human peripheral blood CD14+ monocytes that can differentiate into several distinct mesenchymal cell lineages. We named this cell population monocyte-derived mesenchymal progenitor (MOMP). MOMPs were obtained in vitro from human peripheral blood mononuclear cells cultured on fibronectin in the presence of fetal bovine serum alone as a source of growth factors. MOMPs had a unique molecular phenotype-CD14 +CD45+CD34+type I collagen+-and showed mixed morphologic and molecular features of monocytes and endothelial and mesenchymal cells. MOMPs were found to be derived from a subset of circulating CD14+ monocytes, and their differentiation required that they bind fibronectin and be exposed to one or more soluble factors derived from peripheral blood CD14- cells. MOMPs could be expanded in culture without losing their original phenotype for up to five passages. The induction of MOMPs to differentiate along multiple limb-bud mesodermal lineages resulted in the expression of genes and proteins specific for osteoblasts, skeletal myoblasts, chondrocytes, and adipocytes. Our findings represent the first evidence that human circulating CD14+ monocytes are a source of progenitors that exhibit mesenchymal cell differentiation.

Original languageEnglish
Pages (from-to)833-845
Number of pages13
JournalJournal of Leukocyte Biology
Volume74
Issue number5
DOIs
Publication statusPublished - 2003 Nov
Externally publishedYes

Fingerprint

Monocytes
Cell Differentiation
Fibronectins
Blood Cells
Skeletal Myoblasts
Phenotype
Limb Buds
Cell Lineage
Phagocytes
Chondrocytes
Collagen Type I
Osteoblasts
Adipocytes
Dendritic Cells
Intercellular Signaling Peptides and Proteins
Endothelial Cells
Fibroblasts
Macrophages
Serum
Population

Keywords

  • Fibronectin
  • Lineage
  • Mesenchymal stem
  • Plasticity

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kuwana, M., Okazaki, Y., Kodama, H., Izumi, K., Yasuoka, H., Ogawa, Y., ... Ikeda, Y. (2003). Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation. Journal of Leukocyte Biology, 74(5), 833-845. https://doi.org/10.1189/jlb.0403170

Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation. / Kuwana, Masataka; Okazaki, Yuka; Kodama, Hiroaki; Izumi, Keisuke; Yasuoka, Hidekata; Ogawa, Yoko; Kawakami, Yutaka; Ikeda, Yasuo.

In: Journal of Leukocyte Biology, Vol. 74, No. 5, 11.2003, p. 833-845.

Research output: Contribution to journalArticle

Kuwana, M, Okazaki, Y, Kodama, H, Izumi, K, Yasuoka, H, Ogawa, Y, Kawakami, Y & Ikeda, Y 2003, 'Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation', Journal of Leukocyte Biology, vol. 74, no. 5, pp. 833-845. https://doi.org/10.1189/jlb.0403170
Kuwana, Masataka ; Okazaki, Yuka ; Kodama, Hiroaki ; Izumi, Keisuke ; Yasuoka, Hidekata ; Ogawa, Yoko ; Kawakami, Yutaka ; Ikeda, Yasuo. / Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation. In: Journal of Leukocyte Biology. 2003 ; Vol. 74, No. 5. pp. 833-845.
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