Human Herpesvirus 6 Reactivation Evaluated by Digital Polymerase Chain Reaction and Its Association With Dynamics of CD134-Positive T Cells After Allogeneic Hematopoietic Stem Cell Transplantation

Hitomi Nakayama, Rie Yamazaki, Jun Kato, Yuya Koda, Masatoshi Sakurai, Ryohei Abe, Shintaro Watanuki, Chieko Sumiya, Kohei Shiroshita, Shinya Fujita, Kentaro Yamaguchi, Shinichiro Okamoto, Takehiko Mori

Research output: Contribution to journalArticle


BACKGROUND: Human herpesvirus 6 (HHV-6) causes life-threatening central nervous system disorders after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Recent studies implicated CD134 as a specific receptor of HHV-6B and demonstrated that its expression levels in CD4-positive T cells after allo-HSCT could be related to the reactivation of HHV-6. We prospectively evaluated the relationship between HHV-6 reactivation and CD134+ T cells in the recipients of allo-HSCT. METHODS: HHV-6 viral load in plasma was quantitatively measured weekly after allo-HSCT by digital polymerase chain reaction in 34 patients. The ratio of CD134 in CD4+ T cells (CD134/CD4 ratio) was serially measured by flow cytometry before and after transplantation. RESULTS: HHV-6 reactivation was detected in 23 patients (68%). The CD134/CD4 ratio before conditioning was significantly higher in patients with HHV-6 reactivation than in those without (median, 3.8% vs 1.5%, P < .01). In multivariate analysis, a higher CD134/CD4 ratio before conditioning was significantly associated with the incidence of HHV-6 reactivation (odds ratio, 10.5 [95% confidence interval, 1.3-85.1], P = .03). CONCLUSIONS: A higher CD134/CD4 ratio before conditioning was associated with a higher risk of HHV-6 reactivation, suggesting that the rate may be a promising marker for predicting HHV-6 reactivation after allo-HSCT.

Original languageEnglish
Pages (from-to)1001-1007
Number of pages7
JournalThe Journal of infectious diseases
Issue number6
Publication statusPublished - 2019 Aug 9



  • allogeneic hematopoietic stem cell transplantation
  • CD134
  • digital polymerase chain reaction
  • human herpesvirus 6
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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