Human induced pluripotent stem (iPS) cells-derived neural stem cell bundle covered with growth factor-encapsulated amphiphilic chitosan

Midori Kato-Negishi, Hiroaki Onoe, Shintaroh Iwanaga, Yoshiomi Kobayashi, Masaya Nakamura, Hideyuki Okano, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes human induced pluripotent stem (iPS) cell-derived neural stem cell (hiPSC-NSC) bundles covered with growth factor-encapsulated amphiphilic chitosan. The hiPSC-NSC bundle is formed by 4-12 hiPSC-NSC microfibers that are made by core-shell hydrogel microfibers encapsulating hiPSC-NSCs. The hiPSC-NSC microfibers can be cultured for over two weeks and induced to differentiate into neurons predominantly. We modified the coating material to make bundle structures that have growth factor enrichment capability (Figure 1). The coating material, amphiphilic chitosan, can release growth factors slowly for a long time, allowing hiPSC-NSCs to survive, grow and differentiate into neural lineages in the bundle structure. We believe that our hiPSC-NSC bundle would be an extremely effective tool for neural transplantation in neurodegenerative diseases such as stroke, spinal cord injury and peripheral nerve injury, because hiPSC-NSCs in the bundle structure have long-term survival ability in vivo.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1054-1056
Number of pages3
Volume2
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Chitosan
Stem cells
Intercellular Signaling Peptides and Proteins
Neurodegenerative diseases
Coatings
Hydrogel
Hydrogels
Neurons

Keywords

  • Human induced pluripotent stem cell
  • Neural stem cell
  • Neuron

ASJC Scopus subject areas

  • Bioengineering

Cite this

Kato-Negishi, M., Onoe, H., Iwanaga, S., Kobayashi, Y., Nakamura, M., Okano, H., & Takeuchi, S. (2013). Human induced pluripotent stem (iPS) cells-derived neural stem cell bundle covered with growth factor-encapsulated amphiphilic chitosan. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 2, pp. 1054-1056). Chemical and Biological Microsystems Society.

Human induced pluripotent stem (iPS) cells-derived neural stem cell bundle covered with growth factor-encapsulated amphiphilic chitosan. / Kato-Negishi, Midori; Onoe, Hiroaki; Iwanaga, Shintaroh; Kobayashi, Yoshiomi; Nakamura, Masaya; Okano, Hideyuki; Takeuchi, Shoji.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. p. 1054-1056.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kato-Negishi, M, Onoe, H, Iwanaga, S, Kobayashi, Y, Nakamura, M, Okano, H & Takeuchi, S 2013, Human induced pluripotent stem (iPS) cells-derived neural stem cell bundle covered with growth factor-encapsulated amphiphilic chitosan. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 2, Chemical and Biological Microsystems Society, pp. 1054-1056, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.
Kato-Negishi M, Onoe H, Iwanaga S, Kobayashi Y, Nakamura M, Okano H et al. Human induced pluripotent stem (iPS) cells-derived neural stem cell bundle covered with growth factor-encapsulated amphiphilic chitosan. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2. Chemical and Biological Microsystems Society. 2013. p. 1054-1056
Kato-Negishi, Midori ; Onoe, Hiroaki ; Iwanaga, Shintaroh ; Kobayashi, Yoshiomi ; Nakamura, Masaya ; Okano, Hideyuki ; Takeuchi, Shoji. / Human induced pluripotent stem (iPS) cells-derived neural stem cell bundle covered with growth factor-encapsulated amphiphilic chitosan. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. pp. 1054-1056
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