Human intestinal epithelial cell-derived interleukin (IL)-18, along with IL2, IL-7 and IL-15, is a potent synergistic factor for the proliferation of intraepithelial lymphocytes

A. Okazawa, T. Kanai, K. Nakamaru, T. Sato, N. Inoue, H. Ogata, Y. Iwao, M. Ikeda, T. Kawamura, S. Makita, K. Uraushihara, R. Okamoto, M. Yamazaki, M. Kurimoto, H. Ishii, M. Watanabe, T. Hibi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Intestinal epithelial cell (IEC)-derived cytokines, such as stem cell factor (SCF), interleukin (IL)-7 and IL-15 are known to be required for the development of intestinal intraepithelial lymphocytes (IELs). A newly described cytokine, IL-18, has also been shown to be produced by intestinal epithelial cells. To demonstrate the functional effects of IL-18 on human IELs, we assessed IL-18/IL-18 receptor expression in IEC/IEL and proliferation following stimulation of intestinal IELs by IL-18. IL-18 transcripts were detected both in freshly isolated human colonie epithelial cells and in various colonie epithelial cell lines. IL-18 protein was also detected by ELISA and flow cytometric analysis using antihuman IL-18-specific monoclonal antibody (MoAb). Furthermore, IELs constitutively expressed the IL-18 receptor in addition to the IL-2 and IL-7 receptors. More importantly, IL-18 augmented significant proliferative responses of IEL in combination with IL-2, IL-7 and IL-15 both in the presence and in absence of anti-CD3 MoAb. These results suggest that IL-18 might play a crucial role in the proliferation and maintenance of intestinal IELs.

Original languageEnglish
Pages (from-to)269-276
Number of pages8
JournalClinical and Experimental Immunology
Volume136
Issue number2
DOIs
Publication statusPublished - 2004 May 1

Keywords

  • Human intestinal epithelial cell
  • IL-18
  • Intraepithelial lymphocytes
  • Proliferation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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