Human intestinal epithelial cell-derived interleukin (IL)-18, along with IL2, IL-7 and IL-15, is a potent synergistic factor for the proliferation of intraepithelial lymphocytes

A. Okazawa, Takanori Kanai, K. Nakamaru, Toshiro Sato, Nagamu Inoue, Haruhiko Ogata, Yasushi Iwao, M. Ikeda, T. Kawamura, S. Makita, K. Uraushihara, R. Okamoto, M. Yamazaki, M. Kurimoto, H. Ishii, M. Watanabe, T. Hibi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Intestinal epithelial cell (IEC)-derived cytokines, such as stem cell factor (SCF), interleukin (IL)-7 and IL-15 are known to be required for the development of intestinal intraepithelial lymphocytes (IELs). A newly described cytokine, IL-18, has also been shown to be produced by intestinal epithelial cells. To demonstrate the functional effects of IL-18 on human IELs, we assessed IL-18/IL-18 receptor expression in IEC/IEL and proliferation following stimulation of intestinal IELs by IL-18. IL-18 transcripts were detected both in freshly isolated human colonie epithelial cells and in various colonie epithelial cell lines. IL-18 protein was also detected by ELISA and flow cytometric analysis using antihuman IL-18-specific monoclonal antibody (MoAb). Furthermore, IELs constitutively expressed the IL-18 receptor in addition to the IL-2 and IL-7 receptors. More importantly, IL-18 augmented significant proliferative responses of IEL in combination with IL-2, IL-7 and IL-15 both in the presence and in absence of anti-CD3 MoAb. These results suggest that IL-18 might play a crucial role in the proliferation and maintenance of intestinal IELs.

Original languageEnglish
Pages (from-to)269-276
Number of pages8
JournalClinical and Experimental Immunology
Volume136
Issue number2
DOIs
Publication statusPublished - 2004 May

Fingerprint

Interleukin-15
Interleukin-7
Interleukin-18
Interleukin-2
Epithelial Cells
Lymphocytes
Interleukin-18 Receptors
Interleukin-7 Receptors
Monoclonal Antibodies
Cytokines
Stem Cell Factor
Enzyme-Linked Immunosorbent Assay
Maintenance
Cell Line

Keywords

  • Human intestinal epithelial cell
  • IL-18
  • Intraepithelial lymphocytes
  • Proliferation

ASJC Scopus subject areas

  • Immunology

Cite this

Human intestinal epithelial cell-derived interleukin (IL)-18, along with IL2, IL-7 and IL-15, is a potent synergistic factor for the proliferation of intraepithelial lymphocytes. / Okazawa, A.; Kanai, Takanori; Nakamaru, K.; Sato, Toshiro; Inoue, Nagamu; Ogata, Haruhiko; Iwao, Yasushi; Ikeda, M.; Kawamura, T.; Makita, S.; Uraushihara, K.; Okamoto, R.; Yamazaki, M.; Kurimoto, M.; Ishii, H.; Watanabe, M.; Hibi, T.

In: Clinical and Experimental Immunology, Vol. 136, No. 2, 05.2004, p. 269-276.

Research output: Contribution to journalArticle

Okazawa, A. ; Kanai, Takanori ; Nakamaru, K. ; Sato, Toshiro ; Inoue, Nagamu ; Ogata, Haruhiko ; Iwao, Yasushi ; Ikeda, M. ; Kawamura, T. ; Makita, S. ; Uraushihara, K. ; Okamoto, R. ; Yamazaki, M. ; Kurimoto, M. ; Ishii, H. ; Watanabe, M. ; Hibi, T. / Human intestinal epithelial cell-derived interleukin (IL)-18, along with IL2, IL-7 and IL-15, is a potent synergistic factor for the proliferation of intraepithelial lymphocytes. In: Clinical and Experimental Immunology. 2004 ; Vol. 136, No. 2. pp. 269-276.
@article{8074822d3bce4502929f2ec5fe45fa27,
title = "Human intestinal epithelial cell-derived interleukin (IL)-18, along with IL2, IL-7 and IL-15, is a potent synergistic factor for the proliferation of intraepithelial lymphocytes",
abstract = "Intestinal epithelial cell (IEC)-derived cytokines, such as stem cell factor (SCF), interleukin (IL)-7 and IL-15 are known to be required for the development of intestinal intraepithelial lymphocytes (IELs). A newly described cytokine, IL-18, has also been shown to be produced by intestinal epithelial cells. To demonstrate the functional effects of IL-18 on human IELs, we assessed IL-18/IL-18 receptor expression in IEC/IEL and proliferation following stimulation of intestinal IELs by IL-18. IL-18 transcripts were detected both in freshly isolated human colonie epithelial cells and in various colonie epithelial cell lines. IL-18 protein was also detected by ELISA and flow cytometric analysis using antihuman IL-18-specific monoclonal antibody (MoAb). Furthermore, IELs constitutively expressed the IL-18 receptor in addition to the IL-2 and IL-7 receptors. More importantly, IL-18 augmented significant proliferative responses of IEL in combination with IL-2, IL-7 and IL-15 both in the presence and in absence of anti-CD3 MoAb. These results suggest that IL-18 might play a crucial role in the proliferation and maintenance of intestinal IELs.",
keywords = "Human intestinal epithelial cell, IL-18, Intraepithelial lymphocytes, Proliferation",
author = "A. Okazawa and Takanori Kanai and K. Nakamaru and Toshiro Sato and Nagamu Inoue and Haruhiko Ogata and Yasushi Iwao and M. Ikeda and T. Kawamura and S. Makita and K. Uraushihara and R. Okamoto and M. Yamazaki and M. Kurimoto and H. Ishii and M. Watanabe and T. Hibi",
year = "2004",
month = "5",
doi = "10.1111/j.1365-2249.2004.02431.x",
language = "English",
volume = "136",
pages = "269--276",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Human intestinal epithelial cell-derived interleukin (IL)-18, along with IL2, IL-7 and IL-15, is a potent synergistic factor for the proliferation of intraepithelial lymphocytes

AU - Okazawa, A.

AU - Kanai, Takanori

AU - Nakamaru, K.

AU - Sato, Toshiro

AU - Inoue, Nagamu

AU - Ogata, Haruhiko

AU - Iwao, Yasushi

AU - Ikeda, M.

AU - Kawamura, T.

AU - Makita, S.

AU - Uraushihara, K.

AU - Okamoto, R.

AU - Yamazaki, M.

AU - Kurimoto, M.

AU - Ishii, H.

AU - Watanabe, M.

AU - Hibi, T.

PY - 2004/5

Y1 - 2004/5

N2 - Intestinal epithelial cell (IEC)-derived cytokines, such as stem cell factor (SCF), interleukin (IL)-7 and IL-15 are known to be required for the development of intestinal intraepithelial lymphocytes (IELs). A newly described cytokine, IL-18, has also been shown to be produced by intestinal epithelial cells. To demonstrate the functional effects of IL-18 on human IELs, we assessed IL-18/IL-18 receptor expression in IEC/IEL and proliferation following stimulation of intestinal IELs by IL-18. IL-18 transcripts were detected both in freshly isolated human colonie epithelial cells and in various colonie epithelial cell lines. IL-18 protein was also detected by ELISA and flow cytometric analysis using antihuman IL-18-specific monoclonal antibody (MoAb). Furthermore, IELs constitutively expressed the IL-18 receptor in addition to the IL-2 and IL-7 receptors. More importantly, IL-18 augmented significant proliferative responses of IEL in combination with IL-2, IL-7 and IL-15 both in the presence and in absence of anti-CD3 MoAb. These results suggest that IL-18 might play a crucial role in the proliferation and maintenance of intestinal IELs.

AB - Intestinal epithelial cell (IEC)-derived cytokines, such as stem cell factor (SCF), interleukin (IL)-7 and IL-15 are known to be required for the development of intestinal intraepithelial lymphocytes (IELs). A newly described cytokine, IL-18, has also been shown to be produced by intestinal epithelial cells. To demonstrate the functional effects of IL-18 on human IELs, we assessed IL-18/IL-18 receptor expression in IEC/IEL and proliferation following stimulation of intestinal IELs by IL-18. IL-18 transcripts were detected both in freshly isolated human colonie epithelial cells and in various colonie epithelial cell lines. IL-18 protein was also detected by ELISA and flow cytometric analysis using antihuman IL-18-specific monoclonal antibody (MoAb). Furthermore, IELs constitutively expressed the IL-18 receptor in addition to the IL-2 and IL-7 receptors. More importantly, IL-18 augmented significant proliferative responses of IEL in combination with IL-2, IL-7 and IL-15 both in the presence and in absence of anti-CD3 MoAb. These results suggest that IL-18 might play a crucial role in the proliferation and maintenance of intestinal IELs.

KW - Human intestinal epithelial cell

KW - IL-18

KW - Intraepithelial lymphocytes

KW - Proliferation

UR - http://www.scopus.com/inward/record.url?scp=11144355024&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144355024&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2249.2004.02431.x

DO - 10.1111/j.1365-2249.2004.02431.x

M3 - Article

VL - 136

SP - 269

EP - 276

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 2

ER -