Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition

Masayuki Fujii, Mami Matano, Kohta Toshimitsu, Ai Takano, Yohei Mikami, Shingo Nishikori, Shinya Sugimoto, Toshiro Sato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cellular diversity that shapes tissue architecture and function is governed by multiple niche signals. Nonetheless, maintaining cellular diversity in human intestinal organoids has been challenging. Based on niche ligands present in the natural stem cell milieu, we establish a refined organoid culture condition for intestinal epithelia that allows human intestinal organoids to concurrently undergo multi-differentiation and self-renewal. High-throughput screening reveals that the combination of insulin-like growth factor 1 (IGF-1) and fibroblast growth factor 2 (FGF-2) enhances the clonogenic capacity and CRISPR-genome engineering efficiency of human intestinal stem cells. The combination equally enables long-term culture of a range of intestinal organoids, including rat small intestinal organoids. Droplet-based single-cell RNA sequencing further illustrates the conservation of the native cellular diversity in human small intestinal organoids cultured with the refined condition. The modified culture protocol outperforms the conventional method and offers a viable strategy for modeling human intestinal tissues and diseases in an in vivo relevant context. Sato and colleagues develop a modified culture condition for human intestinal organoids that improves the culture efficiency and maintains their long-term multi-differentiation capacity. scRNA-seq of human small intestinal crypts and organoids demonstrates that in vivo cellular diversity can be preserved in organoids cultured with the refined condition.

Original languageEnglish
Pages (from-to)787-793.e6
JournalCell Stem Cell
Volume23
Issue number6
DOIs
Publication statusPublished - 2018 Dec 6

Fingerprint

Organoids
Small Cytoplasmic RNA
Stem Cells
Clustered Regularly Interspaced Short Palindromic Repeats
RNA Sequence Analysis
Efficiency
Intestinal Diseases
Human Engineering
Somatomedins
Fibroblast Growth Factor 2
Intestinal Mucosa
Genome
Ligands

Keywords

  • CRISPR-Cas9
  • diffusion pseudotime
  • enteroendocrine cells
  • intestinal stem cells
  • LGR5
  • M cells
  • p38 signaling
  • Paneth cells
  • tuft cells
  • ulcerative colitis

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology

Cite this

Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition. / Fujii, Masayuki; Matano, Mami; Toshimitsu, Kohta; Takano, Ai; Mikami, Yohei; Nishikori, Shingo; Sugimoto, Shinya; Sato, Toshiro.

In: Cell Stem Cell, Vol. 23, No. 6, 06.12.2018, p. 787-793.e6.

Research output: Contribution to journalArticle

Fujii, Masayuki ; Matano, Mami ; Toshimitsu, Kohta ; Takano, Ai ; Mikami, Yohei ; Nishikori, Shingo ; Sugimoto, Shinya ; Sato, Toshiro. / Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition. In: Cell Stem Cell. 2018 ; Vol. 23, No. 6. pp. 787-793.e6.
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