Human osteoblasts support hematopoietic cell development in vitro

Yusuke Shiozawa; Hisami Takenouchi; Tomoko Taguchi; Masahiro Saito; Yohko U. Katagiri; Hajime Okita; Toshiaki Shimizu; Yuichiro Yamashiro; Junichiro Fujimoto; Nobutaka Kiyokawa

doi:10.1159/000178144

Human osteoblasts support hematopoietic cell development in vitro

Yusuke Shiozawa, Hisami Takenouchi, Tomoko Taguchi, Masahiro Saito, Yohko U. Katagiri, Hajime Okita, Toshiaki Shimizu, Yuichiro Yamashiro, Junichiro Fujimoto, Nobutaka Kiyokawa

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background/Aim: Although osteoblasts are thought to be the major component of the hematopoietic stem cell niche in the bone marrow microenvironment, the role of osteoblasts in hematopoiesis is still unclear. The ability of human osteoblasts to support early hematopoiesis was investigated. Methods and Results: Human CD34+ bone marrow cells cultured on human osteoblasts were capable of surviving without addition of cytokines and differentiated into myeloid cells with slight proliferation. The results of immunohistochemical experiments suggested activation of FAK and AKT in hematopoietic cells attached to osteoblasts. When stem cell factor, Flt3-L, and IL-3 were added to the coculture system, each cytokine distinctively enhanced proliferation and differentiation of CD34+ bone marrow cells. Conclusion: The results suggest that human osteoblasts have the ability to support hematopoietic cell development in vitro.

Original language	English
Pages (from-to)	134-145
Number of pages	12
Journal	Acta Haematologica
Volume	120
Issue number	3
DOIs	https://doi.org/10.1159/000178144
Publication status	Published - 2009 Jan 1
Externally published	Yes

Keywords

Cell adhesion molecule
Cell signaling
Cytokines
Hematopoiesis
Osteoblasts
Stem cell factor

ASJC Scopus subject areas

Hematology

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title = "Human osteoblasts support hematopoietic cell development in vitro",

abstract = "Background/Aim: Although osteoblasts are thought to be the major component of the hematopoietic stem cell niche in the bone marrow microenvironment, the role of osteoblasts in hematopoiesis is still unclear. The ability of human osteoblasts to support early hematopoiesis was investigated. Methods and Results: Human CD34+ bone marrow cells cultured on human osteoblasts were capable of surviving without addition of cytokines and differentiated into myeloid cells with slight proliferation. The results of immunohistochemical experiments suggested activation of FAK and AKT in hematopoietic cells attached to osteoblasts. When stem cell factor, Flt3-L, and IL-3 were added to the coculture system, each cytokine distinctively enhanced proliferation and differentiation of CD34+ bone marrow cells. Conclusion: The results suggest that human osteoblasts have the ability to support hematopoietic cell development in vitro.",

keywords = "Cell adhesion molecule, Cell signaling, Cytokines, Hematopoiesis, Osteoblasts, Stem cell factor",

author = "Yusuke Shiozawa and Hisami Takenouchi and Tomoko Taguchi and Masahiro Saito and Katagiri, {Yohko U.} and Hajime Okita and Toshiaki Shimizu and Yuichiro Yamashiro and Junichiro Fujimoto and Nobutaka Kiyokawa",

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doi = "10.1159/000178144",

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T1 - Human osteoblasts support hematopoietic cell development in vitro

AU - Shiozawa, Yusuke

AU - Takenouchi, Hisami

AU - Taguchi, Tomoko

AU - Saito, Masahiro

AU - Katagiri, Yohko U.

AU - Okita, Hajime

AU - Shimizu, Toshiaki

AU - Yamashiro, Yuichiro

AU - Fujimoto, Junichiro

AU - Kiyokawa, Nobutaka

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Background/Aim: Although osteoblasts are thought to be the major component of the hematopoietic stem cell niche in the bone marrow microenvironment, the role of osteoblasts in hematopoiesis is still unclear. The ability of human osteoblasts to support early hematopoiesis was investigated. Methods and Results: Human CD34+ bone marrow cells cultured on human osteoblasts were capable of surviving without addition of cytokines and differentiated into myeloid cells with slight proliferation. The results of immunohistochemical experiments suggested activation of FAK and AKT in hematopoietic cells attached to osteoblasts. When stem cell factor, Flt3-L, and IL-3 were added to the coculture system, each cytokine distinctively enhanced proliferation and differentiation of CD34+ bone marrow cells. Conclusion: The results suggest that human osteoblasts have the ability to support hematopoietic cell development in vitro.

AB - Background/Aim: Although osteoblasts are thought to be the major component of the hematopoietic stem cell niche in the bone marrow microenvironment, the role of osteoblasts in hematopoiesis is still unclear. The ability of human osteoblasts to support early hematopoiesis was investigated. Methods and Results: Human CD34+ bone marrow cells cultured on human osteoblasts were capable of surviving without addition of cytokines and differentiated into myeloid cells with slight proliferation. The results of immunohistochemical experiments suggested activation of FAK and AKT in hematopoietic cells attached to osteoblasts. When stem cell factor, Flt3-L, and IL-3 were added to the coculture system, each cytokine distinctively enhanced proliferation and differentiation of CD34+ bone marrow cells. Conclusion: The results suggest that human osteoblasts have the ability to support hematopoietic cell development in vitro.

KW - Cell adhesion molecule

KW - Cell signaling

KW - Cytokines

KW - Hematopoiesis

KW - Osteoblasts

KW - Stem cell factor

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DO - 10.1159/000178144

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JO - Acta Haematologica

JF - Acta Haematologica

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Human osteoblasts support hematopoietic cell development in vitro

Abstract

Keywords

ASJC Scopus subject areas

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