Human osteoblasts support hematopoietic cell development in vitro

Yusuke Shiozawa, Hisami Takenouchi, Tomoko Taguchi, Masahiro Saito, Yohko U. Katagiri, Hajime Okita, Toshiaki Shimizu, Yuichiro Yamashiro, Junichiro Fujimoto, Nobutaka Kiyokawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background/Aim: Although osteoblasts are thought to be the major component of the hematopoietic stem cell niche in the bone marrow microenvironment, the role of osteoblasts in hematopoiesis is still unclear. The ability of human osteoblasts to support early hematopoiesis was investigated. Methods and Results: Human CD34+ bone marrow cells cultured on human osteoblasts were capable of surviving without addition of cytokines and differentiated into myeloid cells with slight proliferation. The results of immunohistochemical experiments suggested activation of FAK and AKT in hematopoietic cells attached to osteoblasts. When stem cell factor, Flt3-L, and IL-3 were added to the coculture system, each cytokine distinctively enhanced proliferation and differentiation of CD34+ bone marrow cells. Conclusion: The results suggest that human osteoblasts have the ability to support hematopoietic cell development in vitro.

Original languageEnglish
Pages (from-to)134-145
Number of pages12
JournalActa Haematologica
Volume120
Issue number3
DOIs
Publication statusPublished - 2009 Jan 1
Externally publishedYes

Keywords

  • Cell adhesion molecule
  • Cell signaling
  • Cytokines
  • Hematopoiesis
  • Osteoblasts
  • Stem cell factor

ASJC Scopus subject areas

  • Hematology

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  • Cite this

    Shiozawa, Y., Takenouchi, H., Taguchi, T., Saito, M., Katagiri, Y. U., Okita, H., Shimizu, T., Yamashiro, Y., Fujimoto, J., & Kiyokawa, N. (2009). Human osteoblasts support hematopoietic cell development in vitro. Acta Haematologica, 120(3), 134-145. https://doi.org/10.1159/000178144