Human plasmacytoid dendritic cells express an atrial natriuretic peptide receptor, guanylyl cyclase-A

Rimpei Morita, Tomoko Fujita, Takashi Uchiyama, Toshiyuki Hori

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Atrial natriuretic peptide is a cardiovascular hormone secreted mainly by the cardiac atria and regulates the volume-pressure homeostasis. The action of ANP is mediated by GC-A.We previously reported that human monocyte-derived dendritic cells express GC-A and respond to ANP with polarization toward a Th2-inducing phenotype. In the present study, we explored the possibility that pDC are subjected to immunoregulation via the ANP/GC-A system.We examined GC-A expression on blood pDC and found that GC-A was not expressed on fresh pDC but was induced after stimulation with CpG-oligodeoxynucleotide AAC-30, IL-3, or interleukin-3 plus CD40 ligand. Activated pDC responded to ANP with an increase in cGMP production, indicating that GC-A expressed on pDC was functional. We investigated whether tonsillar pDC express GC-A by immunohistochemistry and immunofluorescence staining. We found that GC-A+ HLA-DR+ cells were present in the T-cell areas and the perivascular areas. Flow cytometric analysis with tonsillar cells confirmed that lineage- CD123high pDC express GC-A. These results indicate that the ANP/GC-A system is involved in immune regulation through pDC in secondary lymphoid organs.

Original languageEnglish
Pages (from-to)403-411
Number of pages9
JournalMicrobiology and Immunology
Volume53
Issue number7
DOIs
Publication statusPublished - 2009 Jul
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor Receptors
Atrial Natriuretic Factor
Dendritic Cells
Interleukin-3
CD40 Ligand
Oligodeoxyribonucleotides
HLA-DR Antigens
Cell Lineage
Fluorescent Antibody Technique
atrial natriuretic factor receptor A
Monocytes
Homeostasis
Immunohistochemistry
Hormones
Staining and Labeling
T-Lymphocytes
Phenotype
Pressure

Keywords

  • Atrial natriuretic peptide
  • cGMP
  • Interferon-α
  • Plasmacytoid dendritic cells

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology

Cite this

Human plasmacytoid dendritic cells express an atrial natriuretic peptide receptor, guanylyl cyclase-A. / Morita, Rimpei; Fujita, Tomoko; Uchiyama, Takashi; Hori, Toshiyuki.

In: Microbiology and Immunology, Vol. 53, No. 7, 07.2009, p. 403-411.

Research output: Contribution to journalArticle

Morita, Rimpei ; Fujita, Tomoko ; Uchiyama, Takashi ; Hori, Toshiyuki. / Human plasmacytoid dendritic cells express an atrial natriuretic peptide receptor, guanylyl cyclase-A. In: Microbiology and Immunology. 2009 ; Vol. 53, No. 7. pp. 403-411.
@article{92ad50f2ea0e416c879a19a67f3ed8a1,
title = "Human plasmacytoid dendritic cells express an atrial natriuretic peptide receptor, guanylyl cyclase-A",
abstract = "Atrial natriuretic peptide is a cardiovascular hormone secreted mainly by the cardiac atria and regulates the volume-pressure homeostasis. The action of ANP is mediated by GC-A.We previously reported that human monocyte-derived dendritic cells express GC-A and respond to ANP with polarization toward a Th2-inducing phenotype. In the present study, we explored the possibility that pDC are subjected to immunoregulation via the ANP/GC-A system.We examined GC-A expression on blood pDC and found that GC-A was not expressed on fresh pDC but was induced after stimulation with CpG-oligodeoxynucleotide AAC-30, IL-3, or interleukin-3 plus CD40 ligand. Activated pDC responded to ANP with an increase in cGMP production, indicating that GC-A expressed on pDC was functional. We investigated whether tonsillar pDC express GC-A by immunohistochemistry and immunofluorescence staining. We found that GC-A+ HLA-DR+ cells were present in the T-cell areas and the perivascular areas. Flow cytometric analysis with tonsillar cells confirmed that lineage- CD123high pDC express GC-A. These results indicate that the ANP/GC-A system is involved in immune regulation through pDC in secondary lymphoid organs.",
keywords = "Atrial natriuretic peptide, cGMP, Interferon-α, Plasmacytoid dendritic cells",
author = "Rimpei Morita and Tomoko Fujita and Takashi Uchiyama and Toshiyuki Hori",
year = "2009",
month = "7",
doi = "10.1111/j.1348-0421.2009.00149.x",
language = "English",
volume = "53",
pages = "403--411",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "7",

}

TY - JOUR

T1 - Human plasmacytoid dendritic cells express an atrial natriuretic peptide receptor, guanylyl cyclase-A

AU - Morita, Rimpei

AU - Fujita, Tomoko

AU - Uchiyama, Takashi

AU - Hori, Toshiyuki

PY - 2009/7

Y1 - 2009/7

N2 - Atrial natriuretic peptide is a cardiovascular hormone secreted mainly by the cardiac atria and regulates the volume-pressure homeostasis. The action of ANP is mediated by GC-A.We previously reported that human monocyte-derived dendritic cells express GC-A and respond to ANP with polarization toward a Th2-inducing phenotype. In the present study, we explored the possibility that pDC are subjected to immunoregulation via the ANP/GC-A system.We examined GC-A expression on blood pDC and found that GC-A was not expressed on fresh pDC but was induced after stimulation with CpG-oligodeoxynucleotide AAC-30, IL-3, or interleukin-3 plus CD40 ligand. Activated pDC responded to ANP with an increase in cGMP production, indicating that GC-A expressed on pDC was functional. We investigated whether tonsillar pDC express GC-A by immunohistochemistry and immunofluorescence staining. We found that GC-A+ HLA-DR+ cells were present in the T-cell areas and the perivascular areas. Flow cytometric analysis with tonsillar cells confirmed that lineage- CD123high pDC express GC-A. These results indicate that the ANP/GC-A system is involved in immune regulation through pDC in secondary lymphoid organs.

AB - Atrial natriuretic peptide is a cardiovascular hormone secreted mainly by the cardiac atria and regulates the volume-pressure homeostasis. The action of ANP is mediated by GC-A.We previously reported that human monocyte-derived dendritic cells express GC-A and respond to ANP with polarization toward a Th2-inducing phenotype. In the present study, we explored the possibility that pDC are subjected to immunoregulation via the ANP/GC-A system.We examined GC-A expression on blood pDC and found that GC-A was not expressed on fresh pDC but was induced after stimulation with CpG-oligodeoxynucleotide AAC-30, IL-3, or interleukin-3 plus CD40 ligand. Activated pDC responded to ANP with an increase in cGMP production, indicating that GC-A expressed on pDC was functional. We investigated whether tonsillar pDC express GC-A by immunohistochemistry and immunofluorescence staining. We found that GC-A+ HLA-DR+ cells were present in the T-cell areas and the perivascular areas. Flow cytometric analysis with tonsillar cells confirmed that lineage- CD123high pDC express GC-A. These results indicate that the ANP/GC-A system is involved in immune regulation through pDC in secondary lymphoid organs.

KW - Atrial natriuretic peptide

KW - cGMP

KW - Interferon-α

KW - Plasmacytoid dendritic cells

UR - http://www.scopus.com/inward/record.url?scp=70149117749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70149117749&partnerID=8YFLogxK

U2 - 10.1111/j.1348-0421.2009.00149.x

DO - 10.1111/j.1348-0421.2009.00149.x

M3 - Article

VL - 53

SP - 403

EP - 411

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 7

ER -