Human rasGTPase-activating protein (human counterpart of GAP1m): Sequence of the cDNA, primary structure of the protein, production and chromosomal localization

Mariko Kobayashi, Tohru Masui, Jun Kusuda, Yosuke Kameoka, Katsuyuki Hashimoto, Shintaro Iwashita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

When cDNA encoding rat rasGTPase-activating protein (rat GAP1m) was used as a probe, two partial cDNA clones of a human counterpart of rat GAP1m were isolated from a cDNA library derived from growth-arrested normal human ectocervical epithelial cells. One clone was found to be a cDNA of premature mRNA with two introns. A complete cDNA of human GAP1m was constructed by a series of reverse transcription-polymerase chain reaction (RT-PCR) using total RNA from human epidermoid carcinoma A431 cells. Human GAP1m shows 87.7% nucleotide identity to rat GAP1m in open reading frame and encodes an 850-amino acid protein that shows 89.2% identity to rat GAP1m. A 100-kDa protein was detected in A431 cells by Western blotting with anti-rat GAP1m antibody. The human GAP1m gene was mapped to chromosome 3q24-q26.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalGene
Volume175
Issue number1-2
DOIs
Publication statusPublished - 1996 Oct 10

Keywords

  • Bruton's tyrosine kinase homology domain
  • GAP-related domain
  • Human carcinoma A431 cells
  • Human chromosome 3
  • Normal human epithelial cell library
  • mRNA processing

ASJC Scopus subject areas

  • Genetics

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