Human rasGTPase-activating protein (human counterpart of GAP1m): Sequence of the cDNA, primary structure of the protein, production and chromosomal localization

Mariko Kobayashi, Toru Masui, Jun Kusuda, Yosuke Kameoka, Katsuyuki Hashimoto, Shintaro Iwashita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

When cDNA encoding rat rasGTPase-activating protein (rat GAP1m) was used as a probe, two partial cDNA clones of a human counterpart of rat GAP1m were isolated from a cDNA library derived from growth-arrested normal human ectocervical epithelial cells. One clone was found to be a cDNA of premature mRNA with two introns. A complete cDNA of human GAP1m was constructed by a series of reverse transcription-polymerase chain reaction (RT-PCR) using total RNA from human epidermoid carcinoma A431 cells. Human GAP1m shows 87.7% nucleotide identity to rat GAP1m in open reading frame and encodes an 850-amino acid protein that shows 89.2% identity to rat GAP1m. A 100-kDa protein was detected in A431 cells by Western blotting with anti-rat GAP1m antibody. The human GAP1m gene was mapped to chromosome 3q24-q26.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalGene
Volume175
Issue number1-2
DOIs
Publication statusPublished - 1996 Oct 10
Externally publishedYes

Fingerprint

Amino Acid Sequence
Complementary DNA
Proteins
Clone Cells
Gene Library
Introns
Open Reading Frames
Reverse Transcription
Squamous Cell Carcinoma
Nucleotides
Chromosomes
Western Blotting
Epithelial Cells
RNA
Amino Acids
Polymerase Chain Reaction
Messenger RNA
Antibodies
Growth
Genes

Keywords

  • Bruton's tyrosine kinase homology domain
  • GAP-related domain
  • Human carcinoma A431 cells
  • Human chromosome 3
  • mRNA processing
  • Normal human epithelial cell library

ASJC Scopus subject areas

  • Genetics

Cite this

Human rasGTPase-activating protein (human counterpart of GAP1m) : Sequence of the cDNA, primary structure of the protein, production and chromosomal localization. / Kobayashi, Mariko; Masui, Toru; Kusuda, Jun; Kameoka, Yosuke; Hashimoto, Katsuyuki; Iwashita, Shintaro.

In: Gene, Vol. 175, No. 1-2, 10.10.1996, p. 173-177.

Research output: Contribution to journalArticle

Kobayashi, Mariko ; Masui, Toru ; Kusuda, Jun ; Kameoka, Yosuke ; Hashimoto, Katsuyuki ; Iwashita, Shintaro. / Human rasGTPase-activating protein (human counterpart of GAP1m) : Sequence of the cDNA, primary structure of the protein, production and chromosomal localization. In: Gene. 1996 ; Vol. 175, No. 1-2. pp. 173-177.
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