Human SAP is a novel peptidoglycan recognition protein that induces complement-independent phagocytosis of Staphylococcus aureus

Jang Hyun An, Kenji Kurokawa, Dong Jun Jung, Min Jung Kim, Chan Hee Kim, Yukari Fujimoto, Koichi Fukase, K. Mark Coggeshall, Bok Luel Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The human pathogen Staphylococcus aureus is responsible for many community-acquired and hospital-associated infections and is associated with high mortality. Concern over the emergence of multidrug-resistant strains has renewed interest in the elucidation of host mechanisms that defend against S. aureus infection. We recently demonstrated that human serum mannose-binding lectin binds to S. aureus wall teichoic acid (WTA), a cell wall glycopolymer-a discovery that prompted further screening to identify additional serum proteins that recognize S. aureus cell wall components. In this report, we incubated human serum with 10 different S. aureus mutants and determined that serum amyloid P component (SAP) bound specifically to a WTA-deficient S. aureus DtagO mutant, but not to tagO-complemented, WTA-expressing cells. Biochemical characterization revealed that SAP recognizes bacterial peptidoglycan as a ligand and that WTA inhibits this interaction. Although SAP binding to peptidoglycan was not observed to induce complement activation, SAP-bound DtagO cells were phagocytosed by human polymorphonuclear leukocytes in an FcgR-dependent manner. These results indicate that SAP functions as a host defense factor, similar to other peptidoglycan recognition proteins and nucleotide-binding oligomerization domain-like receptors.

Original languageEnglish
Pages (from-to)3319-3327
Number of pages9
JournalJournal of Immunology
Volume191
Issue number6
DOIs
Publication statusPublished - 2013 Sep 15
Externally publishedYes

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Serum Amyloid P-Component
Teichoic Acids
Phagocytosis
Staphylococcus aureus
Peptidoglycan
Cell Wall
Mannose-Binding Lectin
Cytophagocytosis
Complement Activation
Protein S
Community Hospital
Cellular Structures
Cross Infection
Serum
Protein Binding
Blood Proteins
Neutrophils
Nucleotides
peptidoglycan recognition protein
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Human SAP is a novel peptidoglycan recognition protein that induces complement-independent phagocytosis of Staphylococcus aureus. / An, Jang Hyun; Kurokawa, Kenji; Jung, Dong Jun; Kim, Min Jung; Kim, Chan Hee; Fujimoto, Yukari; Fukase, Koichi; Coggeshall, K. Mark; Lee, Bok Luel.

In: Journal of Immunology, Vol. 191, No. 6, 15.09.2013, p. 3319-3327.

Research output: Contribution to journalArticle

An, Jang Hyun ; Kurokawa, Kenji ; Jung, Dong Jun ; Kim, Min Jung ; Kim, Chan Hee ; Fujimoto, Yukari ; Fukase, Koichi ; Coggeshall, K. Mark ; Lee, Bok Luel. / Human SAP is a novel peptidoglycan recognition protein that induces complement-independent phagocytosis of Staphylococcus aureus. In: Journal of Immunology. 2013 ; Vol. 191, No. 6. pp. 3319-3327.
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