Human synovial sarcoma proto-oncogene Syt is essential for early embryonic development through the regulation of cell migration

Taichi Kimura, Mieko Sakai, Kouichi Tabu, Lei Wang, Ryosuke Tsunematsu, Masumi Tsuda, Hirofumi Sawa, Kazuo Nagashima, Hiroshi Nishihara, Shigetsugu Hatakeyama, Keiko Nakayama, Marc Ladanyi, Shinya Tanaka, Keiichi I. Nakayama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

SYT-SSX protein, resulted from chromosomal translocation, causes synovial sarcoma, which is a malignant tumor accounting for 10% of soft tissue sarcoma. However, biological functions of SYT (synovial sarcoma translocation), also known as SS18, are largely unclear, whereas it has been proven that Syt-null mice die at early stages of embryonic development. Here, we generated Syt-deficient mice and confirmed the reported phenotypes, including growth retardation, open neural tube and haplo-insufficient lethality, and therefore, there is no doubt that Syt is essential for embryonic development. However, placental defects, described in the earlier report, were rarely seen in our mice and we frequently observed cardiac defect in Syt-deficient mice. As the mechanisms responsible for embryonic lethality seem to be complicate, we performed additional experiments. By using primary cultured embryonic fibroblasts, we showed that Syt / MEFs deregulate actin organization and suppressed cell migration. These observations suggest that Syt may contribute to the signaling pathway important for various cellular functions in vivo and in vitro, and we propose that Syt-deficient MEFs would be a powerful means to understand the biological roles of SYT in vitro.

Original languageEnglish
Pages (from-to)645-656
Number of pages12
JournalLaboratory Investigation
Volume89
Issue number6
DOIs
Publication statusPublished - 2009 Jun 1
Externally publishedYes

Fingerprint

Synovial Sarcoma
Proto-Oncogenes
Embryonic Development
Cell Movement
Genetic Translocation
Neural Tube
Protein Transport
Sarcoma
Actins
Fibroblasts
Phenotype
Growth
Neoplasms
In Vitro Techniques

Keywords

  • Cardiogenesis
  • Embryonic lethality
  • Motility
  • P300
  • Synovial sarcoma
  • Syt

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Human synovial sarcoma proto-oncogene Syt is essential for early embryonic development through the regulation of cell migration. / Kimura, Taichi; Sakai, Mieko; Tabu, Kouichi; Wang, Lei; Tsunematsu, Ryosuke; Tsuda, Masumi; Sawa, Hirofumi; Nagashima, Kazuo; Nishihara, Hiroshi; Hatakeyama, Shigetsugu; Nakayama, Keiko; Ladanyi, Marc; Tanaka, Shinya; Nakayama, Keiichi I.

In: Laboratory Investigation, Vol. 89, No. 6, 01.06.2009, p. 645-656.

Research output: Contribution to journalArticle

Kimura, T, Sakai, M, Tabu, K, Wang, L, Tsunematsu, R, Tsuda, M, Sawa, H, Nagashima, K, Nishihara, H, Hatakeyama, S, Nakayama, K, Ladanyi, M, Tanaka, S & Nakayama, KI 2009, 'Human synovial sarcoma proto-oncogene Syt is essential for early embryonic development through the regulation of cell migration', Laboratory Investigation, vol. 89, no. 6, pp. 645-656. https://doi.org/10.1038/labinvest.2009.25
Kimura, Taichi ; Sakai, Mieko ; Tabu, Kouichi ; Wang, Lei ; Tsunematsu, Ryosuke ; Tsuda, Masumi ; Sawa, Hirofumi ; Nagashima, Kazuo ; Nishihara, Hiroshi ; Hatakeyama, Shigetsugu ; Nakayama, Keiko ; Ladanyi, Marc ; Tanaka, Shinya ; Nakayama, Keiichi I. / Human synovial sarcoma proto-oncogene Syt is essential for early embryonic development through the regulation of cell migration. In: Laboratory Investigation. 2009 ; Vol. 89, No. 6. pp. 645-656.
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