Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation

Tomoko Ito, Naoko Iida-Tanaka, Takuro Niidome, Takahito Kawano, Koji Kubo, Kenichi Yoshikawa, Toshinori Sato, Zhihong Yang, Yoshiyuki Koyama

Research output: Contribution to journalArticlepeer-review

129 Citations (Scopus)

Abstract

Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.

Original languageEnglish
Pages (from-to)382-388
Number of pages7
JournalJournal of Controlled Release
Volume112
Issue number3
DOIs
Publication statusPublished - 2006 May 30

Keywords

  • HMG
  • Hyaluronic acid
  • Transcription
  • Transfection

ASJC Scopus subject areas

  • Pharmaceutical Science

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