Hyaluronic acid and its derivative as a multi-functional gene expression enhancer

Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation

Tomoko Ito, Naoko Iida-Tanaka, Takuro Niidome, Takahito Kawano, Koji Kubo, Kenichi Yoshikawa, Toshinori Sato, Zhihong Yang, Yoshiyuki Koyama

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.

Original languageEnglish
Pages (from-to)382-388
Number of pages7
JournalJournal of Controlled Release
Volume112
Issue number3
DOIs
Publication statusPublished - 2006 May 30

Fingerprint

Hyaluronic Acid
Polyethyleneimine
Transcriptional Activation
Gene Expression
Plasmids
High Mobility Group Proteins
Spermine
CHO Cells
DNA
Glycosaminoglycans
Transgenes
Transcription Factors
Ligands
Proteins

Keywords

  • HMG
  • Hyaluronic acid
  • Transcription
  • Transfection

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Hyaluronic acid and its derivative as a multi-functional gene expression enhancer : Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation. / Ito, Tomoko; Iida-Tanaka, Naoko; Niidome, Takuro; Kawano, Takahito; Kubo, Koji; Yoshikawa, Kenichi; Sato, Toshinori; Yang, Zhihong; Koyama, Yoshiyuki.

In: Journal of Controlled Release, Vol. 112, No. 3, 30.05.2006, p. 382-388.

Research output: Contribution to journalArticle

Ito, Tomoko ; Iida-Tanaka, Naoko ; Niidome, Takuro ; Kawano, Takahito ; Kubo, Koji ; Yoshikawa, Kenichi ; Sato, Toshinori ; Yang, Zhihong ; Koyama, Yoshiyuki. / Hyaluronic acid and its derivative as a multi-functional gene expression enhancer : Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation. In: Journal of Controlled Release. 2006 ; Vol. 112, No. 3. pp. 382-388.
@article{388fea0f05504a2ba172b9245a326133,
title = "Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation",
abstract = "Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.",
keywords = "HMG, Hyaluronic acid, Transcription, Transfection",
author = "Tomoko Ito and Naoko Iida-Tanaka and Takuro Niidome and Takahito Kawano and Koji Kubo and Kenichi Yoshikawa and Toshinori Sato and Zhihong Yang and Yoshiyuki Koyama",
year = "2006",
month = "5",
day = "30",
doi = "10.1016/j.jconrel.2006.03.013",
language = "English",
volume = "112",
pages = "382--388",
journal = "Journal of Controlled Release",
issn = "0168-3659",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Hyaluronic acid and its derivative as a multi-functional gene expression enhancer

T2 - Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation

AU - Ito, Tomoko

AU - Iida-Tanaka, Naoko

AU - Niidome, Takuro

AU - Kawano, Takahito

AU - Kubo, Koji

AU - Yoshikawa, Kenichi

AU - Sato, Toshinori

AU - Yang, Zhihong

AU - Koyama, Yoshiyuki

PY - 2006/5/30

Y1 - 2006/5/30

N2 - Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.

AB - Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.

KW - HMG

KW - Hyaluronic acid

KW - Transcription

KW - Transfection

UR - http://www.scopus.com/inward/record.url?scp=33646690293&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646690293&partnerID=8YFLogxK

U2 - 10.1016/j.jconrel.2006.03.013

DO - 10.1016/j.jconrel.2006.03.013

M3 - Article

VL - 112

SP - 382

EP - 388

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 3

ER -