Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential

Takuya Shimazaki, Hitoshi Okazawa, Hideta Fujii, Masako Ikeda, Katsuyuki Tamai, Ronald D G McKay, Masami Muramatsu, Hiroshi Hamada

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The Oct-3 gene is expressed in highly undifferentiated cells and is implicated in mammalian early embryogenesis. We have generated a series of hybrid cells between pluripotent embryonal carcinoma cells (Oct-3+) and fibroblasts (Oct-3-), and have studied the regulation and function of Oct-3. Upon fusion, the hybrid cells differentiated to nestin+/Brn-2+ cells resembling neuroepithelial stem cells. Expression of Oct-3 was extinguished at the transcriptional level in all the hybrid cells examined. The Oct-3 modulating activity required for the Oct-3-mediated enhancer activation was also extinguished. When the Oct-3 transactivating function was introduced into the hybrid cells, they transformed into morphologically distinct nestin-/Brn-2- cells Crevertants'). When the 'revertant' cells subsequently lost Oct-3 expression, they differentiated back to nestin+/Brn-2+ cells. The close correlation between the phenotypic changes and the gain/loss of Oct-3 function indicates that Oct-3 can induce dedifferentiation of the neural cells.

Original languageEnglish
Pages (from-to)4489-4498
Number of pages10
JournalEMBO Journal
Volume12
Issue number12
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Nestin
Hybrid Cells
Fibroblasts
Stem cells
Cell Dedifferentiation
Neuroepithelial Cells
Embryonal Carcinoma Stem Cells
Fusion reactions
Genes
Chemical activation
Cells
Embryonic Development
Stem Cells

Keywords

  • Embryogenesis
  • Hybrid cell extinction
  • Oct-3
  • POU

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Shimazaki, T., Okazawa, H., Fujii, H., Ikeda, M., Tamai, K., McKay, R. D. G., ... Hamada, H. (1993). Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential. EMBO Journal, 12(12), 4489-4498.

Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential. / Shimazaki, Takuya; Okazawa, Hitoshi; Fujii, Hideta; Ikeda, Masako; Tamai, Katsuyuki; McKay, Ronald D G; Muramatsu, Masami; Hamada, Hiroshi.

In: EMBO Journal, Vol. 12, No. 12, 1993, p. 4489-4498.

Research output: Contribution to journalArticle

Shimazaki, T, Okazawa, H, Fujii, H, Ikeda, M, Tamai, K, McKay, RDG, Muramatsu, M & Hamada, H 1993, 'Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential', EMBO Journal, vol. 12, no. 12, pp. 4489-4498.
Shimazaki T, Okazawa H, Fujii H, Ikeda M, Tamai K, McKay RDG et al. Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential. EMBO Journal. 1993;12(12):4489-4498.
Shimazaki, Takuya ; Okazawa, Hitoshi ; Fujii, Hideta ; Ikeda, Masako ; Tamai, Katsuyuki ; McKay, Ronald D G ; Muramatsu, Masami ; Hamada, Hiroshi. / Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential. In: EMBO Journal. 1993 ; Vol. 12, No. 12. pp. 4489-4498.
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