Hybridization between Japanese and North American Chlamydomonas reinhardtii (Volvocales, Chlorophyceae)

Takashi Nakada, Yudai Tsuchida, Kazuharu Arakawa, Takuro Ito, Masaru Tomita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The microalga Chlamydomonas reinhardtii is a model organism whose whole genome has been sequenced. Although considered a cosmopolitan species, only eastern North American isolates of C. reinhardtii were available before 2010, when new Japanese isolates were reported. In the study describing the new Japanese isolates, zygote formation between Japanese and North American strains was shown, but germination was not demonstrated. In this study, the germination of intercontinental hybrid zygotes was examined using wild-type Japanese strains and mutant American strains that cannot utilize nitrate. Several clonal progeny strains were established, and the progeny strains were screened based on mating type and nitrate utilization to confirm their hybrid nature. The establishment of four intercontinental hybrid strains with different phenotypic combinations was confirmed by sequencing mating type-specific and nitrate reductase-related genes. The potential for hybrid formation between Japanese and North American strains suggests the existence of a worldwide mating population of C. reinhardtii.

Original languageEnglish
Pages (from-to)232-236
Number of pages5
JournalPhycological Research
Volume62
Issue number3
DOIs
Publication statusPublished - 2014 Jul

Keywords

  • Biogeography
  • Chlamydomonas reinhardtii
  • Chlorophyceae
  • Mating population
  • Species

ASJC Scopus subject areas

  • Aquatic Science
  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

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